Download PTU (I.K. Gujral Punjab Technical University Jalandhar (IKGPTU) B.Sc-Agriculture 2020 March Previous Question Papers
1 | M-77102 (S2)-2553
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc. (Agriculture) (Sem.?7)
PLANT TISSUE CULTURE AND TRANSFORMATION
Subject Code : BSAG-PGB-706
M.Code : 77102
Time : 3 Hrs. Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.
SECTION-A
Q1. Write briefly :
a) Growth Regulators with example.
b) Artificial seeds.
c) LMOs.
d) Somaclonal Variants.
e) Gelling Agents.
f) Transient and Stable transgene expression.
g) Explant and its Sterilization.
h) Expression Vectors with example.
i) Pollen Culture and Ovary Culture .
j) Golden rice.
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1 | M-77102 (S2)-2553
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc. (Agriculture) (Sem.?7)
PLANT TISSUE CULTURE AND TRANSFORMATION
Subject Code : BSAG-PGB-706
M.Code : 77102
Time : 3 Hrs. Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.
SECTION-A
Q1. Write briefly :
a) Growth Regulators with example.
b) Artificial seeds.
c) LMOs.
d) Somaclonal Variants.
e) Gelling Agents.
f) Transient and Stable transgene expression.
g) Explant and its Sterilization.
h) Expression Vectors with example.
i) Pollen Culture and Ovary Culture .
j) Golden rice.
2 | M-77102 (S2)-2553
SECTION-B
Q2. What are the risks posed by transgene to Human health and Environment?
Q3. a) Define Reporter genes and its types with examples.
b) What are the features of an ideal reporter gene?
c) Out of Hygromycin phosphotransferase and Green fluorescent protein gene which is
a scorable marker gene.
Q4. By which technique, the copy number of a transgene could be detected? Explain its
principle briefly.
Q5. Genetic engineering of crop plants contributes towards foods for the future. Explain.
Q6. a) Define Transgene and Transgenics.
b) List any 4 good laboratory practices that need to be taken care of during genetic
transformation experiment.
c) List any 4 commercialized transgenic crop plants.
SECTION-C
Q7. a) Enlist various approaches for gene transformation.
b) Describe in details about particle gun mediated plant transformation. What are its
advantages and limitations over Agrobacterium mediated transformation approaches.
Q8. a) How Genetic engineering contributed towards production of Insect resistant plants?
b) What is the principle behind conducting bioassays with transgenic plants?
Q9. Define Biosafety. Enlist the name and functions of the competent authorities of National
Biosafety Regulatory Framework governing Transgenics in India.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on
any page of Answer Sheet will lead to UMC against the Student.
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This post was last modified on 02 April 2020