Download PTU B.Sc-Agriculture 2020 March 7th Sem 77103 Molecular Biotechnology And Genomics Question Paper

Download PTU (I.K. Gujral Punjab Technical University Jalandhar (IKGPTU) B.Sc-Agriculture 2020 March Previous Question Papers

1 | M-77103 (S2)-2588
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc. (Agriculture) (Sem.?7)
MOLECULAR BIOTECHNOLOGY AND GENOMICS
Subject Code : BSAG-PGB-707
M.Code : 77103
Time : 3 Hrs. Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
Q1. Write briefly :
a) Genome
b) BLAST and its types
c) pBR322
d) Palindromic sequences
e) Shuttle Vectors
f) LOD score
g) NCBI
h) Isoschizomers with example
i) ISSRs
j) Linkers and Adaptors

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1 | M-77103 (S2)-2588
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc. (Agriculture) (Sem.?7)
MOLECULAR BIOTECHNOLOGY AND GENOMICS
Subject Code : BSAG-PGB-707
M.Code : 77103
Time : 3 Hrs. Max. Marks : 60
INSTRUCTIONS TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
Q1. Write briefly :
a) Genome
b) BLAST and its types
c) pBR322
d) Palindromic sequences
e) Shuttle Vectors
f) LOD score
g) NCBI
h) Isoschizomers with example
i) ISSRs
j) Linkers and Adaptors

2 | M-77103 (S2)-2588
SECTION-B
Q2. a) Define Gene Cloning and enlist its basic steps.
b) What are the functions of the following in a gene cloning experiment?
i) Type II endonucleases
ii) Alkaline phosphatases
Q3. a) Define RFLPs. What is the basis of polymorphism using RFLP markers?
b) What do you understand by polymorphic and monomorphic marker?
c) Are SSRs dominant or co-dominant markers?
Q4. Define Proteome. What are the major steps that constitute a proteome analysis?
Q5. What is the principle of Sanger?s sequencing method and what are its advantages over
Maxam and Gilbert sequencing technique?
Q6. Define Molecular map. Discuss briefly about the various segregating populations that
could be used to construct a linkage map.

SECTION-C
Q7. Discuss in details about the Principle, Advantages, Limitations and Applications of any
two Hybridization techniques.
Q8. What is a cDNA clone? Enlist basic steps involved in cDNA cloning. Describe in details
the synthesis of first and second strand of cDNA.
Q9. a) Define PCR and its various components. Diagrammatically represent different stages
of PCR.
b) Name any two bioinformatics tools for PCR primers designing.
c) Give any three reasons that could lead to PCR failure.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on
any page of Answer Sheet will lead to UMC against the Student.
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