Download PTU B.Sc-BT 2020 March 4th Sem 47049 Recombinant Dna Technology Question Paper

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Roll No. [ ] Total No. of Pages : 02
Total No. of Questions : 09

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B.Sc.(BT) (2014 to 2017) (Sem.-4)
RECOMBINANT DNA TECHNOLOGY
Subject Code : BSBT-206
M.Code : 47049

Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

SECTION-A

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Q1. Define :

1. Linkers and adapters.
2. Sticky ends and flush ends
3. Expression vectors
4. Nested PCR

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5. Chromosome walking
6. Molecular maps
7. Cosmids
8. Human genome project
9. Targeted gene therapy

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10. Homopolymeric tailing

SECTION-B

Q2. What are the types and properties of restriction enzymes?

Q3. Write a note on artificial chromosomes.

Q4. Discuss technique for PCR product analysis.

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Q5. Write a note on yeast vectors.

Q6. List properties of a good vector.

SECTION-C

Q7. Elaborate the steps involved in Genomic Library Construction.

Q8. What are molecular markers? Discuss different types and their application.

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Q9. Write about different types and applications of PCR and its limitations.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.