Download PTU B.Sc-BT 2020 March 5th Sem 47056 Animal Cell Culture And Biotechnology Question Paper

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Roll No.

Total No. of Questions : 09

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B.Sc.(BT) (2014 to 2017) (Sem.-5)

ANIMAL CELL CULTURE AND BIOTECHNOLOGY

Subject Code : BSBT-301

M.Code : 47056

Time : 3 Hrs.

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Max. Marks : 60

INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

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SECTION-A

Q1. Define briefly :

1. Monolayer culture
2. Histotypic culture
3. Hybrid Antibodies

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4. Cell Cultures
5. Cell line
6. Biolistic gun method
7. Recombinant proteins
8. Generation number

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9. Transfection
10. In vitro fertilization

SECTION-B

Q2. Differentiate between Suspension and Monolayer cultures.

Q3. Write a note on immobilized cultures.

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Q4. Discuss steps in initiation of primary culture.

Q5. Write a note on Embryo transfer.

Q6. Discuss about natural and artificial media used for cell culture.

SECTION-C

Q7. Elaborate on production of monoclonal antibodies.

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Q8. What are transgenic animals? Discuss various methods of transfection.

Q9. Write about bioethical considerations and guidelines for cell culture research.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.

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