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Seat No.: Enrolment No.
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GUJARAT TECHNOLOGICAL UNIVERSITY
BE- SEMESTER-VII (NEW) EXAMINATION - WINTER 2020
Subject Code:2170401 Date:19/01/2021
Subject Name:Enzymes and Proteins
Time:10:30 AM TO 12:30 PM Total Marks: 56
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Instructions:
- Attempt any FOUR questions out of EIGHT questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
MARKS
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Q.1 (a) Explain the role of entropy in catalysis of enzyme. 03
(b) Explain about specificity of enzymes. 04
(c) Explain the method of classification of enzyme. 07
Q.2 (a) Discuss the significance of Vmax. 03
(b) Explain features and working of active site. 04
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(c) Compare different plots and modifications used to overcome limitations of Michealis-Menten plot with equation. 07Q.3 (a) What do you mean by enthalpy, entropy and free energy. 03
(b) Write Michaelis-Menten equation & Write assumptions for derivation of Michaelis-Menten equation. 04
(c) Explain the concept of standard free energy. 07
Q.4 (a) Write a note on Sanger's method for sequencing genes. 03
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(b) What are various features of protein protein interface? 04(c) Discuss in detail about the factors that affect the rate of chemical reaction. 07
Q.5 (a) Which method can give the highest resolution of protein structure? Write a brief note about the same. 03
(b) Compare NMR and X-Ray crystallography as protein structure tools. 04
(c) Write a note on CD-Spectroscopy, Maldi-Tof and at least one method for understanding protein primary structure. 07
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Q.6 (a) Write note with examples on enzymes from plant source, animal source and microbial source. 03
(b) Write a note about commercial applications of enzymes in at least two different industries. 04
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(c) Write a note on allosteric activation and inhibition as a means for control of protein activity. 07Q.7 (a) Define enzyme activity, effectiveness factor and domains. 03
(b) What is Anfinsen’s hypothesis? How it was demonstrated? 04
(c) Write a note on enzyme immobilization. 07
Q.8 (a) Effect of denaturants on protein structure. 03
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(b) How folding funnels describe folding phenomenon? 04(c) Explain GroEL GroES system and its function. 07
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