Download GTU B.Pharma 2020 Winter 7th Sem BP701TP Instrumental Method of Analysis Question Paper

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1. Attempt any THREE questions from Q-1 to Q-6.
2. Q.7 is compulsory to attempt.
3. Make suitable assumptions wherever necessary.
4. Figures to the right indicate full marks.

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1. Derive Beer’s Law. Give reasons for deviations from Beer’s law? (06)
2. Define Chromophores, auxochromes and Bathochromic shift with example. (05)
3. What is chemical quenching? Give its example. (05)

1. Explain sample handling in IR spectroscopy. (06)
2. Describe Principle and instrumentation of Flame photometry. (05)

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3. Describe thermal detectors used in IR spectrophotometry. (05)

1. Describe principle involved in separation in TLC along with stationary Phases used in TLC. (06)
2. Give advantages and disadvantages of Adsorption chromatography (05)
3. What are the modes of development in paper chromatography? (05)

1. Write a note on Gel electrophoresis along with its applications. (06)

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2. Give applications of Thin Layer Chromatography. (05)
3. Describe principle and applications of Atomic absorption spectroscopy (05)

1. What is the principle and which carrier gas is suitable for use in a. Katharometer b. Flame ionization detector c. Electron capture detector (06)
2. Differentiate between isocratic and gradient elution technique. (05)
3. Define Retention time, Retention Volume, Resolution and HETP. (05)

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1. What is an Ion exchange resin? (06)
2. Write factors affecting ion exchange chromatography (05)
3. Give application of Gel and affinity chromatography. (05)

1. Which principle is involved in Normal Phase and Reverse Phase chromatography (06)
   OR
   

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2. What are the different types of vibrations? Explain in detail (06)
   OR
   
3. Explain Instrumentation of UV-Visible spectrophotometer (06)

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