Download GTU B.Pharma 2020 Winter 5th Sem 2250002 Pharmaceutical Microbiology And Biotechnology I Question Paper

Download GTU (Gujarat Technological University Ahmedabad) B.Pharma (Bachelor of Pharmacy) 2020 Winter 5th Sem 2250002 Pharmaceutical Microbiology And Biotechnology I Previous Question Paper

Seat No.: _____ Enrolment No. _____________
GUJARAT TECHNOLOGICAL UNIVERSITY
B.Pharm - SEMESTER?V ? EXAMINATION ? WINTER -2020
Subject Code:2250002
Date: 05/01/2021
Subject Name: Pharmaceutical Microbiology &Biotechnology-I
Time: 10:30AM TO 12:30PM
Total Marks: 54
Instructions:


1. Attempt any THREE questions from Q-1 to Q-6.
2. Q.7 is compulsory to attempt.
3. Make suitable assumptions wherever necessary.
4. Figures to the right indicate full marks.

Q.1 (a) Define microbiology. Discuss the various scope of microbiology.
06

(b) Discuss principle, working and applications of TEM.
05

(c) Differentiate: Prokaryotes and eukaryotes, Bacteria and virus.
05



Q.2 (a) Give the suitable condition for growth of following: Autotrophs,
06
Chemolithotrophs, Microaerophiles, Psychrophiles, Basophiles, Obligates
anaerobes.

(b) Differentiate: spore and capsule, Gm+ve and Gm ?ve bacteria.
05

(c) Write a note on bacterial flagella.
05



Q.3 (a) Define and Classify culture media. Write a note on Enriched media.
06

(b) What are FTM and SCM? Write a note on aerobic and anaerobic media.
05

(c) Define total count and viable count. Discuss any one method used for viable
05
counting of E.coli.



Q.4 (a) Explain D-value, F-value, Z-value, Q10Value, SAL, Bio burden.
06

(b) Write a note on Cold sterilization methods.
05

(c) Comment on followings: (i) Superheated steam is more active than saturated
05
steam. (ii) Roll tube method cannot be used for viable count of anaerobic
organism.



Q.5 (a) Suggest suitable sterilization method for Oily injections, Boric acid,
06
Transfusion sets, Petri plates, Ethyl oleate and Syringes.

(b) Differentiate: tyndallization and pasteurization, Sterilization and disinfection.
05

(c) Write a note on biological indicators for monitoring of sterilization.
05



Q. 6 (a) Enlist the various evaluation tests for disinfectant. Write a note on Riedel
06
Walker coefficient test.

(b) Write a note on QAC and halide class of disinfectants.
05

(c) Discuss principle and procedure involved in staining for mycobacterium
05
species.



Q.7 (a) Define immobilization. Enlist methods of immobilization and write any one in
06
detail.
OR

(a) Discuss in detail about genetic code and its properties
06
OR

(a) Write a note on DNA replication.
06
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