Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
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B.Pharm - SEMESTER- VI EXAMINATION — SUMMER -2020
Subject Code: BPS04TP Date: 29-10-2020
Subject Name: Pharmaceutical Microbiology
Time: 2:30 PM TO 5:30 PM Total Marks: 80
Instructions:
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- Attempt any five questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
Q.1 (a) How is preparation of microbial specimen done for compound microscopy? 06
(b) Write a brief note on (any one) 05
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- Phase contrast Microscopy
- Fluorescence Microscopy
- Electron Microscopy
(c) What are Differential stains? Write on principle and procedure citing a suitable example. 05
Q.2 (a) Draw a labeled diagram of a Prokaryotic cell. 06
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(b) Write on (any two) 05
- Cell wall of Gram positive and Gram negative cell
- Endospores
- Ribosomes
(c) Give differences between a Eukaryotic cell and a Prokaryotic cell. 05
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Q.3 (a) Write on different Nutrient media in details. 06
(b) Give methods of Preservation of bacterial cultures. 05
(c) Explain methods of Direct measurement of microbial growth. 05
Q.4 (a) Enumerate Physical methods of microbial control and write on Autoclaving with a labeled diagram. 06
(b) Classify Disinfectants: Enumerate their methods of evaluation. Write on Ethylene oxide as chemo sterilizer. 05
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(c) Write in brief on (any one) 05
- Filtration sterilization
- Radiation sterilization
Q.5 (a) Write on defects (any one) 06
- Viruses
- Classification of Fungi
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(b) Give objective and Pharmacopoeial method for Sterility testing of Water for Injection. 05
(c) Write on various methods of Microbiological Assay explaining standardization of an antibiotic. 05
Q.6 (a) Write on: Laminar flow equipments 06
(b) Write on: Different sources of contamination 05
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(c) Write on: Clean area classification 05
Q.7 (a) What are called Microbial Spoilages? Give their types. Write on factors affecting spoilage of pharmaceutical products by microbes. 06
(b) Differentiate between a Bacteriostatic and a Bactericidal agent giving suitable examples 05
(c) Write on Biochemical tests for identification and classification of bacteria. 05
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