Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
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B.PHARM - SEMESTER- 6 EXAMINATION - SUMMER -2020
Subject Code: 2260002 Date:27-10-2020
Subject Name: Pharmaceutical Microbiology & Biotechnology — 11
Time: 10:30 AM TO 1:30 PM Total Marks: 80
Instructions:
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- Attempt any five questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
Q.1 (a) Define and classify fermentation with suitable examples. Enumerate various 06 methods of fermentation.
(b) Write short notes on fermentative production of Streptomycin. 05
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(c) Give a brief account on recovery methods of product from the fermented broth. 05
Q.2 (a) Explain the microbiological assay of Streptomycin. 06
(b) Write short notes on Test for sterility. 05
(c) Give a brief account on Oral Polio vaccine. 05
Q.3 (a) i)Define: Immunity, Virulence, Attenuation, Toxoid, Exotoxin, Endotoxin 06
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ii) State the Enzymes associated with Virulence.(b) Write short notes on Schick Test. 05
(c) How to carry out the Microbiological assay of Vitamin B1. 05
Q.4 (a) Enumerate the various steps involved in Monoclonal Antibody Production. 06
(b) Give a brief account on rDNA technology. 05
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(c) Define: Transformation, Conjugation, Transduction, Protoplast fusion, 05 Electrophoration.
Q.5 (a) Explain the vaccine prepared by rDNA technology. 06
(b) Define Mutation. What are the types and consequences of mutation? 05
(c) List out the tests for identifying the mutants and explain any one. 05
Q.6 (a) Describe the method of preparing DPT vaccine. 06
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(b) Explain the I.P. standards to be followed for BCG vaccine preparation. 05
(c) Write short notes on anticoagulants used in blood products with their merits and demerits. 05
Q.7 (a) Explain the method of preparation of Dried Human Plasma. 06
(b) Enumerate Dextran as Plasma substitute. 05
(c) Write short notes on Human Plasma Protein fraction. 05
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