Download GTU B.Pharma 2019 Winter 5th Sem 2250002 Pharmaceutical Microbiology And Biotechnology I Question Paper

Download GTU (Gujarat Technological University Ahmedabad) B.Pharma (Bachelor of Pharmacy) 2019 Winter 5th Sem 2250002 Pharmaceutical Microbiology And Biotechnology I Previous Question Paper

Seat No.: _____ Enrolment No. _____________
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM - SEMESTER? 5 EXAMINATION ? WINTER -2019
Subject Code: 2250002 Date: 18-11-2019
Subject Name: Pharmaceutical Microbiology & Biotechnology ? I
Time: 02:30 PM TO 05:30 PM
Total Marks: 80

Instructions:


1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1
(a) Describe growth curve of bacteria with labeled diagram.
06

(b) Classify the staining techniques. Discuss gram staining method.
05

(c)
Draw a labeled diagram of bacterial cell. Explain flagella.
05



Q.2
(a) Write a brief note on nutritional requirements of microorganisms.
06

(b) Classify media. Write in detail about any one media used for
05
cultivation of anaerobic organisms.

(c)
Define the terms: Bioburden, Sterilization, D- value, Incineration, MIC.
05



Q.3
(a) Define sterilization. Classify various methods of sterilization. Write in brief
06
about Hot Air Oven.

(b) How will you perform the sterility test for penicillin?
05

(c)
Write a note on gaseous sterilization.
05



Q.4
(a) Describe the structure of DNA and the process of DNA replication.
06

(b) Discuss in detail about genetic code and its properties.
05

(c)
Give a brief account on restriction endonucleases.
05



Q.5
(a) Define enzyme immobilization. Write merits and demerits of enzyme
06
immobilization.

(b) Discuss preservation of bacterial cultures.
05

(c)
Differentiate total and viable count. Explain the methods of determining total
05
count.



Q. 6
(a) Enumerate the factors affecting disinfection. Discuss any two.
06

(b) Enlist the methods of evaluation of disinfectant. Discuss phenol coefficient
05
test.

(c)
Explain Redeal walker coefficient test in comparison to Chick martin test.
05



Q.7
(a) Write detail note on spirochetes.
06

(b) Describe biological indicators for monitoring sterilization processes.
05

(c)
Write applications of biotechnology in Pharmacy.
05
***************

---