Download GTU B.Pharma 2019 Winter 5th Sem BP504TP Pharmaceutical Microbiology Question Paper

Download GTU (Gujarat Technological University Ahmedabad) B.Pharma (Bachelor of Pharmacy) 2019 Winter 5th Sem BP504TP Pharmaceutical Microbiology Previous Question Paper

Seat No.: _____ Enrolment No. _____________
GUJARAT TECHNOLOGICAL UNIVERSITY
B. PHARM - SEMESTER? V ? EXAMINATION ? WINTER -2019
Subject Code: BP504TP
Date: 22/11/2019
Subject Name: Pharmaceutical Microbiology
Time: 02:30PM TO 05:30PM
Total Marks: 80

Instructions:


1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1
(a) Classify bacteria according to their physical requirement for the growth with suitable
06
example

(b) Differentiate following
05
i) Gram positive and Gram negative bacteria
ii) Bacteria and virus

(c)
Enlist types of media for bacterial growth with suitable example. Explain selective and
05
differential media



Q.2
(a) Draw a labeled diagram of horizontal autoclave and discuss its principal, merits,
06
demerits and applications.

(b) What is differential staining technique? explain acid fast staining
05

(c)
Discuss cultivation of anaerobic bacteria in detail
05



Q.3
(a) Classify the disinfectants. Enlist the factors affecting disinfection. Discuss any two
06

(b) Enumerate the methods of evaluation of disinfectant. Explain Rideal-Walkar test
05

(c)
Explain various methods for the preservation of pure culture
05



Q.4
(a) Explain significance of control test, Media and Method recommended for the sterility
06
testing of Pharmaceutical product as per IP

(b) Give a detailed account on Gaseous sterilization
05

(c)
Discuss sterility indicators for the validation of Sterilization methods
05



Q.5
(a) Enumerate various methods used for microbiological Assay. Discuss one level assay
06
method for microbial assay of antibiotics

(b) How do you carried out the microbiological assay of Vitamin B12 as per IP
05

(c)
Explain electron microscopy with its application and limitation
05



Q. 6
(a) Enumerate technique for the quantitative measurement of bacterial growth and
06
explain any one method for viable count

(b) Write a short not on IMViC test for the identification of bacteria
05

(c)
Discuss factors affecting the microbial spoilage of Pharmaceutical products
05



Q.7
(a) Explain the various sources of contamination in an aseptic area and methods of
06
prevention

(b) Give the applications of cell cultures in Pharmaceutical industry
05

(c)
Explain various parameters to maximize the preservative efficacy of an antimicrobial
05
agent
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