Download GTU B.Pharma 2019 Winter 5th Sem BP504TP Pharmaceutical Microbiology Question Paper

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Seat No.: Enrolment No.

GUJARAT TECHNOLOGICAL UNIVERSITY

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Instructions:

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1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

1. (a) Classify bacteria according to their physical requirement for the growth with suitable example [06]
   (b) Differentiate following [05]
   

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   i) Gram positive and Gram negative bacteria
   ii) Bacteria and virus
   (c) Enlist types of media for bacterial growth with suitable example. Explain selective and differential media [05]
2. (a) Draw a labeled diagram of horizontal autoclave and discuss its principal, merits, demerits and applications. [06]
   (b) What is differential staining technique? explain acid fast staining [05]
   

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   (c) Discuss cultivation of anaerobic bacteria in detail [05]
3. (a) Classify the disinfectants. Enlist the factors affecting disinfection. Discuss any two [06]
   (b) Enumerate the methods of evaluation of disinfectant. Explain Rideal-Walkar test [05]
   (c) Explain various methods for the preservation of pure culture [05]
4. (a) Explain significance of control test, Media and Method recommended for the sterility testing of Pharmaceutical product as per IP [06]
   

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   (b) Give a detailed account on Gaseous sterilization [05]
   (c) Discuss sterility indicators for the validation of Sterilization methods [05]
5. (a) Enumerate various methods used for microbiological Assay. Discuss one level assay method for microbial assay of antibiotics [06]
   (b) How do you carried out the microbiological assay of Vitamin B12 as per IP [05]
   (c) Explain electron microscopy with its application and limitation [05]

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6. (a) Enumerate technique for the quantitative measurement of bacterial growth and explain any one method for viable count [06]
   (b) Write a short not on IMVIC test for the identification of bacteria [05]
   (c) Discuss factors affecting the microbial spoilage of Pharmaceutical products [05]
7. (a) Explain the various sources of contamination in an aseptic area and methods of prevention [06]
   (b) Give the applications of cell cultures in Pharmaceutical industry [05]
   

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   (c) Explain various parameters to maximize the preservative efficacy of an antimicrobial agent [05]

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