Download GTU B.Pharma 2019 Summer 5th Sem 2250002 Pharmaceutical Microbiology And Biotechnology I Question Paper

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Enrolment No. ______

GUJARAT TECHNOLOGICAL UNIVERSITY

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B.PHARM - SEMESTER- 5 EXAMINATION - SUMMER -2019

Subject Code: 2250002 Date: 29-05-2019

Subject Name: Pharmaceutical Microbiology & Biotechnology — I

Time: 02:30 PM TO 05:30 PM Total Marks: 80

Instructions:

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1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1

1. Define sterilization and classify the methods with mechanisms of action & name of the instrument used. Explain the autoclave. (06)

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2. Give a brief account on mechanical method of sterilization. (05)
3. Write short notes on physical, chemical and biological indicators for checking the efficiency of autoclave (05)

Q.2

1. Classify the staining techniques. Discuss gram staining method. (06)
2. Differentiate bacteria and viruses. (05)

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3. Write application and scope of Pharmaceutical Microbiology. (05)

Q.3

1. Enlist various evaluation tests for disinfectants, Describe the Rideal Walker test for phenol. Write its merits & demerits. (06)
2. Define Disinfectant. Discuss in detail ideal properties of disinfectant. (05)
3. Define the following terms: Bioburden, Antiseptic, Thermal death point, Bactericide, Sanitizer (05)

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Q.4

1. Classify methods of bacterial count. Differentiate between Total count and Viable count methods. Discuss plate count method of counting bacteria in detail. (06)
2. Draw and discuss in detail normal bacterial growth curve (05)
3. Classify bacteria according to their shape and size. Write functions of capsule and fimbriae. (05)

Q.5

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1. Explain salient features of enzyme immobilization. (06)
2. Discuss any two methods of immobilization of enzymes. (05)
3. Write note on bacterial flagella. (05)

Q.6

1. Give a brief account on various staining techniques to identify microbes. (06)

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2. Classify the bacteria with respect to oxygen requirement, flagellar arrangement and temperature requirement. (05)
3. Write short notes on oxygen toxicity to anaerobic organisms. (05)

Q.7

1. Discuss in detail about various techniques used for maintenance and preservation of pure culture. (06)
2. Write a detailed note on the scope of Biotechnology. (05)

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3. Discuss in detail about translation. (05)

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