Download GTU B.Pharma 2018 Summer 5th Sem 2250002 Pharmaceutical Microbiology And Biotechnology I Question Paper

Download GTU (Gujarat Technological University) B.Pharma (Bachelor of Pharmacy) 2018 Summer 5th Sem 2250002 Pharmaceutical Microbiology And Biotechnology I Previous Question Paper

Seat No.: _____ Enrolment No. _____________
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharm - SEMESTER?5 ? EXAMINATION ? SUMMER -2018
Subject Code: 2250002 Date: 16/05/2018
Subject Name: Pharmaceutical Microbiology & Biotechnology - I
Time: 02:30 PM TO 05:30 PM
Total Marks: 80
Instructions:


1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) Enlist the methods of evaluation of disinfectant. Discuss phenol coefficient test. 06

(b) Differentiate: Gram +ve Bacteria and Gram ?ve Bacteria, Bacteria and Virus.
05

(c) Write a note on TEM.
05



Q.2 (a) Enlist the different methods of bacterial counting. Write a note on one of viable
06
counting method.

(b) Write a short note on flagella and capsule.
05

(c) Define terms: D-Value, Z-Value, Bioburden, Sterility, MIC
05



Q.3 (a) Enumerate various techniques of enzyme immobilization and explain any one
06
in detail.

(b) Write a note on dark field microscopy.
05

(c) Discuss the scope of biotechnology in pharmacy.
05



Q.4 (a) Classify Bacterial Identification methods. Discuss principle involved in Zeil-
06
neelson Staining.

(b) (i) Comment: Oily injections in a sealed container can be sterilized by
05
autoclave.
(ii) Comment: Oxygen is lethal or life for microorganisms.

(c) Write a brief note on Protein synthesis.
05



Q.5 (a) Write a note on DNA Replication.
06

(b) Define sterility assurance.Write a note on Biological indicators.
05

(c) Write a note on Rickettsia and Actinomycetes.
05



Q. 6 (a) Define: Autotrophs, Chemotrophs, Microaerophiles, Psychrophile, Facultative
06
anaerobes, chemolithotrophs, Capinophiles.

(b) Differentiates: Prokaryotes and Eucaryotes
05
Differentiates: DNA and RNA.

(c) Enlist factor affecting disinfection.Discuss any two in detail.
05



Q.7 (a) Classify Culture media.Discuss in detail any two culture media.
06

(b) Suggest suitable sterilization method for: Arachis oil, Boric acid, Surgical
05
gloves, Glass beaker, Ascorbic acid injection.

(c) Give mechanism of killing: Ethylene oxide sterilization, Pasteurization, Oil
05
bath sterilization, UV radiation, Surface sterilization method.
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