Seat No.:
Subject Code: 2250002
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Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharm - SEMESTER-5 EXAMINATION - SUMMER -2018
Subject Name: Pharmaceutical Microbiology & Biotechnology - I
Time: 02:30 PM TO 05:30 PM
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Total Marks: 80
Date: 16/05/2018
Instructions:
- Attempt any five questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
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- (a) Enlist the methods of evaluation of disinfectant. Discuss phenol coefficient test. (06)
(b) Differentiate: Gram +ve Bacteria and Gram -ve Bacteria, Bacteria and Virus. (05)
(c) Write a note on TEM. (05) - (a) Enlist the different methods of bacterial counting. Write a note on one of viable counting method. (06)
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(b) Write a short note on flagella and capsule. (05)
(c) Define terms: D-Value, Z-Value, Bioburden, Sterility, MIC (05) - (a) Enumerate various techniques of enzyme immobilization and explain any one in detail. (06)
(b) Write a note on dark field microscopy. (05)
(c) Discuss the scope of biotechnology in pharmacy. (05) - (a) Classify Bacterial Identification methods. Discuss principle involved in Zeil-neelson Staining. (06)
(b) (i) Comment: Oily injections in a sealed container can be sterilized by autoclave. (05)
(ii) Comment: Oxygen is lethal or life for microorganisms. (05) - (a) Write a brief note on Protein synthesis. (06)
(b) Write a note on DNA Replication. (05)--- Content provided by FirstRanker.com ---
(c) Define sterility assurance. Write a note on Biological indicators. (05) - (a) Write a note on Rickettsia and Actinomycetes. (06)
(b) Define: Autotrophs, Chemotrophs, Microaerophiles, Psychrophile, Facultative anaerobes, chemolithotrophs, Capinophiles. (05)
(c) Differentiates: Prokaryotes and Eucaryotes (05) - (a) Differentiates: DNA and RNA. (06)
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(b) Enlist factor affecting disinfection. Discuss any two in detail. (05)
(c) Classify Culture media. Discuss in detail any two culture media. (05) - (a) Suggest suitable sterilization method for: Arachis oil, Boric acid, Surgical gloves, Glass beaker, Ascorbic acid injection. (06)
(b) Give mechanism of killing: Ethylene oxide sterilization, Pasteurization, Oil bath sterilization, UV radiation, Surface sterilization method. (05)
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