Seat No.:
Subject Code: 2260002
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Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
B. Pharm. - SEMESTER-6 * EXAMINATION — SUMMER -2018
Subject Name: Pharmaceutical Microbiology & Biotechnology - I1
Time: 10:30 AM TO 01:30 PM
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Instructions:
- Attempt any five questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
- (a) Explain microbiological assay of Vitamin B12. 06
- (b) Discuss the isolation & recovery of Fermentation products. 05
- (c) Enlist methods for sterility testing. Discuss direct inoculation method. 05
- (a) Differentiate the sera and vaccines with examples. 06
- (b) Write short notes on 1) Streptokinase i1) Monoclonal antibodies 05
- (c) Write in detail the fermentative productions of ethanol. 05
- (a) Describe the methods for Standardization & Screening of soil for organisms producing antibiotics. 06
- (b) Give the flow chart for the production of vitamin B12. 05
- (c) What do you understand by the term mutagenesis? Explain Radiation as Mutagen. 05
- (a) Describe scope of Biotechnology & its applications in pharmacy. 06
- (b) Describe ideal characteristics of plasma substituent. Explain Dextran as a plasma substituent. 05
- (c) Write a note on mutation and its consequences. 05
- (a) Explain MoAb production and biochemical principle involved in hybrid selection. 06
- (b) Write a note on recombinant DNA technology. 05
- (c) Define Immunity and Classify it with examples. 05
- (a) Write a note on protoplast Fusion. 06
- (b) How is BCG vaccine prepared & sterilized? 05
- (c) Why ELISA and Widal tests are performed? Explain the Tuberculin test. 05
- (a) Describe antigen-antibody reactions with examples. 06
- (b) Sketch the flow diagrams for recovery & purification of streptomycin and riboflavin from broth. 05
- (c) Give short notes on Immunoglobulins. 05
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Total Marks: 80
Date: 05/05/2018
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