Download PTU B.Sc -MLS 3rd Semester 2019 May 48110 ANALYTICAL BIOCHEMISTRY Question Paper

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Roll No. [ ] Total No. of Pages : 02
Total No. of Questions : 09

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B.Sc.(MLS) (2013 to 2017) (Sem.-3)
ANALYTICAL BIOCHEMISTRY
Subject Code : BMLS-305
M.Code : 48110
Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students has to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students has to attempt any TWO questions.

SECTION-A

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1) Answer briefly :

1. Discuss Lambert’s law.
2. What is a standard?
3. What is a stationary phase?
4. What is monochromatic-light?

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5. What is ground state-of an atom?
6. Discuss the applications of HPLC.
7. Name various parts of a spectrophotometer.
8. What is the significance of a control?
9. What do we mean by void volume?

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10. Discuss principle of gel chromatography.

SECTION-B

2) Write any six applications of electrophoresis and their advantages and disadvantages.

3) Explain principle, method and applications of ion exchange chromatography.

4) Give principle and limitations of Flame photometry.

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5) Write a short note on hydrophobic interactions.

6) Explain principle, applications, advantages and disadvantages of using gas chromatography.

SECTION-C

7) Define Electrophoresis. Explain PAGE and isoelectric focusing in detail.

8) Define Chromatography. Explain principle, application, advantages and disadvantages of using column chromatography.

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9) Explain Beer and Lambert’s law. Write at least six applications of colorimetry and spectrophotometry. Put some words also about advantages of using spectrophotometer.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.