Download PTU B-Tech Bio Technology 2020 Dec 5th Sem 70504 Genetic Engineering Question Paper

Download PTU (I.K.Gujral Punjab Technical University (IKGPTU)) B-Tech (Bachelor of Technology) BT-Bio Technology 2020 December 5th Sem 70504 Genetic Engineering Previous Question Paper

Roll No.
Total No. of Pages : 02
Total No. of Questions : 18
B.Tech. (BT) (2012 to 2017) (Sem.?5)
GENETIC ENGINEERING
Subject Code : BTBT-503
M.Code : 70504
Time : 3 Hrs. Max. Marks : 60
INST RUCT IONS T O CANDIDAT ES :
1 .
SECT ION-A is COMPULSORY cons is ting of TEN questions carrying TWO marks
each.
2 .
SECT ION-B c ontains F IVE questions c arrying FIVE marks eac h and s tud ents
have to atte mpt any FOUR q ues tions.
3 .
SECT ION-C contains THREE questions carrying T EN marks e ach and s tudents
have to atte mpt any T WO questio ns.
SECTION-A
Answer briefly :
1.
What is the role of T4 DNA ligase in cloning?
2.
Explain the technique of Northern blotting.
3.
What are BACs?
4.
Explain the applications of using PCR.
5.
Explain the technique of SI mapping.
6.
What are cosmids? Explain how cosmids are used for cloning?
7.
What is random site directed mutagenesis?
8.
Write a note about agarose gel electrophoresis?
9.
What are restriction Maps?
10. Briefly explain DNA microarrays.
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SECTION-B
11. Comment on cDNA Library versus Genomic DNA Library.
12. Explain the expression of cloned genes in Bacterial systems using suitable examples.
13. Write a note on restriction enzymes, their source, mode of action and production of
cleavage sites.
14. Explain in detail about PCR and RT-PCR.
15. What is Phage Display? Design an experiment for the Selection of mutant peptides by
phage display technique.
SECTION-C
16. a) Explain any two plant expression vector.
b) Explain the screening of recombinant clones.
17. Describe in detail about the physical and chemical methods for transferring DNA into the
host cell.
18. a) What are the factors affecting the expression of cloned genes?
b) Explain the Codon optimization in designing vectors.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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