Download PTU B-Tech Bio Technology 2020 Dec 5th Sem 70505 Animal Cell Culture And Biotechnology Question Paper

Download PTU (I.K.Gujral Punjab Technical University (IKGPTU)) B-Tech (Bachelor of Technology) BT-Bio Technology 2020 December 5th Sem 70505 Animal Cell Culture And Biotechnology Previous Question Paper

Roll No.
Total No. of Pages : 02
Total No. of Questions : 18
B.Tech. (BT) (2012 to 2017) (Sem.?5)
ANIMAL CELL CULTURE & BIOTECHNOLOGY
Subject Code : BTBT-504
M.Code : 70505
Time : 3 Hrs. Max. Marks : 60
INST RUCT IONS T O CANDIDAT ES :
1 .
SECT ION-A is COMPULSORY cons is ting of TEN questions carrying TWO marks
each.
2 .
SECT ION-B c ontains F IVE questions c arrying FIVE marks eac h and s tud ents
have to atte mpt any FOUR q ues tions.
3 .
SECT ION-C contains THREE questions carrying T EN marks e ach and s tudents
have to atte mpt any T WO questio ns.
SECTION-A
Answer briefly :
1.
What is the application of cell culture in area of Toxicology?
2.
What are the properties of Suspension cultures?
3.
Explain the warm Trypsinisation method for primary culture process.
4.
What is a Mycoplasma?
5.
What is gene therapy?
6.
Where is a roller bottle used for?
7.
Explain sub-culturing procedure.
8.
How would you eradicate fungal contamination in animal cell culture?
9.
Where is collagenase used for?
10. Explain clay out of a tissue or cell culture laboratory.
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SECTION-B
11. What are the different types of reactor systems required for large scale animal cell
production?
12. Explain the Embryonic stem cell method for the creation of Transgenics and its
disadvantages.
13. Explain the different methods required to detect mycoplasma contamination in animal
cell culture.
14. Explain what is serum free media? Also write about the advantages and disadvantages of
using serum free media.
15. Write about the biohazards and risk assessment in animal cell culture laboratories.
SECTION-C
16. What are cell lines? How are they developed? How they are different form primary cells?
Give the potential advantages of cell lines over primary cells.
17. Describe how gene therapy has been used for treating human genetic disorders citing
different examples?
18. What is a complete media? Describe the necessary components which are present in a
complete media.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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