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Roll No. : HHHHHH Total No. of Pages : 02
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Total No. of Questions : 18B.Tech. (BT) (2012 to 2017) (Sem.=5)
ANIMAL CELL CULTURE & BIOTECHNOLOGY
Subject Code : BTBT-504
M.Code : 70505
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Time : 3 Hrs. Max. Marks : 60INSTRUCTIONS TO CANDIDATES :
- SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
- SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
- SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.
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SECTION-A
Answer briefly :
- What is the application of cell culture in area of Toxicology?
- What are the properties of Suspension cultures?
- Explain the warm Trypsinisation method for primary culture process.
- What is a Mycoplasma?
- What is gene therapy?
- Where is a roller bottle used for?
- Explain sub-culturing procedure.
- How would you eradicate fungal contamination in animal cell culture?
- Where is collagenase used for?
- Explain clay out of a tissue or cell culture laboratory.
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SECTION-B
- What are the different types of reactor systems required for large scale animal cell production?
- Explain the Embryonic stem cell method for the creation of Transgenics and its disadvantages.
- Explain the different methods required to detect mycoplasma contamination in animal cell culture.
- Explain what is serum free media? Also write about the advantages and disadvantages of using serum free media.
- Write about the biohazards and risk assessment in animal cell culture laboratories.
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SECTION-C
- What are cell lines? How are they developed? How they are different form primary cells? Give the potential advantages of cell lines over primary cells.
- Describe how gene therapy has been used for treating human genetic disorders citing different examples?
- What is a complete media? Describe the necessary components which are present in a complete media.
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NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.
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This download link is referred from the post: PTU B.Tech Question Papers 2020 December (All Branches)