Download PTU B-Tech Bio Technology 2020 Dec 6th Sem 71076 Bioanalytical Techniques Question Paper

Download PTU (I.K.Gujral Punjab Technical University (IKGPTU)) B-Tech (Bachelor of Technology) BT-Bio Technology 2020 December 6th Sem 71076 Bioanalytical Techniques Previous Question Paper

Roll No.
Total No. of Pages : 02
Total No. of Questions : 18
B.Tech. (Biotechnology) (2012 Onwards) (Sem.?6)
BIOANALYTICAL TECHNIQUES
Subject Code : BTBT-605
M.Code : 71076
Time : 3 Hrs. Max. Marks : 60
INST RUCT ION TO CANDIDAT ES :
1 .
SECT ION-A is COMPULSORY cons is ting of TEN questions carrying TWO marks
each.
2 .
SECT ION-B c ontains F IVE questions c arrying FIVE marks eac h and s tud ents
have to atte mpt any FOUR q ues tions.
3 .
SECT ION-C contains THREE questions carrying T EN marks e ach and s tudents
have to atte mpt any T WO questio ns.
SECTION-A
Answer briefly :
1.
What is relative centrifugal force?
2.
Define resolution of a microscope.
3.
What is the importance of SDS in SDS-PAGE?
4.
Define distribution coefficient.
5.
Define Beer-Lambert law.
6.
What is the application of infrared spectroscopy?
7.
Define isotopes.
8.
What is the application of CD spectroscopy?
9.
What type of mobile phase is used in GC?
10. What are the different types of rotors used in centrifugation?
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SECTION-B
11. Differentiate between light microscope and electron microscope.
12. What is autoradiography? How it is performed and what are its applications in biological
sciences?
13. Explain affinity chromatography and its applications.
14. What is the importance of centrifugation in biotechnology? Write a note on differential
centrifugation and its application.
15. What is the principle of mass spectrometry? Explain MALDI-TOF and write its
applications.
SECTION-C
16. Explain functioning of UV visible spectrophotometer with the help of schematic diagram.
Also compare it with spectrofluorimeter and highlight the major difference between the
two instruments.
17. Explain agarose gel electrophoresis? What kinds of molecules are usually analyzed by
agarose gel electrophoresis and how are they visualized in the gel? Comment on
relationship between molecular weight and mobility of the molecules when analyzed in
agarose gel electrophoresis.
18. Explain HPLC. How are samples applied onto a HPLC column? Also comment on the
pumps and detectors used in HPLC.
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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