Download PTU M.Tech. Bio Tech 1st Semester 23005 BIOPROCESS ENGINEERING and TECHNOLOGY Question Paper

Download PTU. I.K. Gujral Punjab Technical University (IKGPTU) M.Tech. Bio Tech 1st Semester 23005 BIOPROCESS ENGINEERING and TECHNOLOGY Question Paper.

1 | M-23005 (S9)-1432

Roll No. Total No. of Pages : 02
Total No. of Questions : 08
M.Tech.(Bio. Tech.) (Sem.?1)
BIOPROCESS ENGINEERING & TECHNOLOGY
Subject Code : MTBT-105
M.Code : 23005
Time : 3 Hrs. Max. Marks : 100

INSTRUCTIONS TO CANDIDATES :
1. Attempt any FIVE questions out of EIGHT questions.
2. Each question carries TWENTY marks.

1. a) Discuss the fixed-volume fed-batch culture system with relevant mass-balance
expressions. (10)
b) Why a fed batch reactor is preferred over CSTR for the commercial production of
biochemical products. (10)
2. a) What is log penetration theory? State its relevance in terms of designing a filter for air
sterilization. (10)
b) Discuss the significance of Del factor in media sterilization. (10)
3. a) Define the term ?volumetric transfer coefficient? in an aerated bioreactor? State its
importance. (10)
b) Discuss the effect of ?power consumption? on K
L
a value of a bioreactor. (10)
4. a) Discuss various controllers that can be used in a bioprocess control system. (10)
b) Explain the working principle of a thermocouple with a neat and well labelled
diagram. (10)
5. a) Discuss the various stages involved while recovering a biochemical product from the
fermentation broth. (10)
b) What are the important criteria for designing a bioseparation processes? Explain with
suitable examples. (10)

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1 | M-23005 (S9)-1432

Roll No. Total No. of Pages : 02
Total No. of Questions : 08
M.Tech.(Bio. Tech.) (Sem.?1)
BIOPROCESS ENGINEERING & TECHNOLOGY
Subject Code : MTBT-105
M.Code : 23005
Time : 3 Hrs. Max. Marks : 100

INSTRUCTIONS TO CANDIDATES :
1. Attempt any FIVE questions out of EIGHT questions.
2. Each question carries TWENTY marks.

1. a) Discuss the fixed-volume fed-batch culture system with relevant mass-balance
expressions. (10)
b) Why a fed batch reactor is preferred over CSTR for the commercial production of
biochemical products. (10)
2. a) What is log penetration theory? State its relevance in terms of designing a filter for air
sterilization. (10)
b) Discuss the significance of Del factor in media sterilization. (10)
3. a) Define the term ?volumetric transfer coefficient? in an aerated bioreactor? State its
importance. (10)
b) Discuss the effect of ?power consumption? on K
L
a value of a bioreactor. (10)
4. a) Discuss various controllers that can be used in a bioprocess control system. (10)
b) Explain the working principle of a thermocouple with a neat and well labelled
diagram. (10)
5. a) Discuss the various stages involved while recovering a biochemical product from the
fermentation broth. (10)
b) What are the important criteria for designing a bioseparation processes? Explain with
suitable examples. (10)

2 | M-23005 (S9)-1432

6. a) Derive the equation for terminal settling velocity for a spherical solid moving under
acceleration due to gravity. (6)
b) How would settling velocity of the same particle change if the rpm of the centrifuges
is increased by 25%?? (4)
c) Explain the working of a tubular bowl centrifuge. (10)
7. a) What is the role of ?precipitation? in downstream processing? (10)
b) Discuss ?salting-in? and ?salting-out? phenomena for protein purification. (10)
8. Write short notes on following :
a) Aqueous two phase extraction (10)
b) Types of control system in bioprocessing (10)









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