Download PTU M.Tech. Bio Tech 2nd Semester 23004 APPLIED BIOTECHNOLOGY Question Paper

Download PTU. I.K. Gujral Punjab Technical University (IKGPTU) M.Tech. Bio Tech 2nd Semester 23004 APPLIED BIOTECHNOLOGY Question Paper.

1 | M-23004 (S9)-770

Roll No. Total No. of Pages : 02
Total No. of Questions : 08
M.Tech. (Bio Tech.) (Sem.?2)
APPLIED BIOTECHNOLOGY
Subject Code : MTBT-104
M.Code : 23004
Time : 3 Hrs. Max. Marks : 100

INSTRUCTION TO CANDIDATES :
1. Attempt any FIVE questions out of EIGHT questions.
2. Each question carries TWENTY marks.

Q1 a) What are restriction enzymes? Give classification of restriction enzyme with suitable
example? (3+4)
b) What is PCR? Give stepwise process of DNA amplification using PCR. (3+10)
Q2 a) Highlight the differences between RT-PCR and Real time PCR in terms of process
and applications. (10)
b) What is a DNA Chip? Discuss the applications of DNA chips in Agriculture and
Medicine. (3+7)
Q3 a) What is northern blotting? Give an account of applications of Northern blotting.
(5+5)
b) What are the main features of the Yeast Artificial Chromosomes (YAC) vectors? (8)
c) What is the importance of a reporter gene? (2)
Q4 a) Write short notes on any three (approx. 500 words) (5 ? 3)
a. Fluorescence In situ hybridization
b. DNA foot printing
c. Cosmids
d. Ex vivo gene therapy
e. Southern Blotting
b) What is anti-sense RNA technology? Highlight applications. (3+2)
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1 | M-23004 (S9)-770

Roll No. Total No. of Pages : 02
Total No. of Questions : 08
M.Tech. (Bio Tech.) (Sem.?2)
APPLIED BIOTECHNOLOGY
Subject Code : MTBT-104
M.Code : 23004
Time : 3 Hrs. Max. Marks : 100

INSTRUCTION TO CANDIDATES :
1. Attempt any FIVE questions out of EIGHT questions.
2. Each question carries TWENTY marks.

Q1 a) What are restriction enzymes? Give classification of restriction enzyme with suitable
example? (3+4)
b) What is PCR? Give stepwise process of DNA amplification using PCR. (3+10)
Q2 a) Highlight the differences between RT-PCR and Real time PCR in terms of process
and applications. (10)
b) What is a DNA Chip? Discuss the applications of DNA chips in Agriculture and
Medicine. (3+7)
Q3 a) What is northern blotting? Give an account of applications of Northern blotting.
(5+5)
b) What are the main features of the Yeast Artificial Chromosomes (YAC) vectors? (8)
c) What is the importance of a reporter gene? (2)
Q4 a) Write short notes on any three (approx. 500 words) (5 ? 3)
a. Fluorescence In situ hybridization
b. DNA foot printing
c. Cosmids
d. Ex vivo gene therapy
e. Southern Blotting
b) What is anti-sense RNA technology? Highlight applications. (3+2)
2 | M-23004 (S9)-770

Q5 Give an account on the role of recombinant DNA technology in production of therapeutic
proteins. (20)
Q6 a) Describe the genetic features of the vector pBR322. (8)
b) What are the salient genetic features of a typical E. coli based expression vector? (8)
c) Explain the meaning of ?Competent E.coli?. (4)
Q7 a) Give the major applications of CDNA and genomic library. (10)
b) Describe the structure of BAC vector. (10)
Q8 a) Define Gene Therapy. How is in vivo gene therapy different from ex vivo gene
therapy? (5+5)
b) Describe the method of production of the transgenic plant for insect resistance. (10)








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