Download Graduate Aptitude Test in Engineering (GATE) 2015 XL I Life Sciences Question Paper
XL-I 1/5
I : BIOCHEMISTRY
Q. 1 ? Q. 10 carry one mark each.
Q.1 Which one of the following small molecules is a prerequisite for fatty acid oxidation?
(A) Inositol (B) Choline (C) Carnitine (D) Glycerol
Q.2 Which one of the following bases is NOT found in the T-arm of an aminoacyl t-RNA?
(A) Dihydrouridine
(B) Pseudouridine
(C) Uracil
(D) Guanine
Q.3 Oxidation of one molecule of glucose via the glycerol-phosphate shuttle produces
(A) 32 molecules of ATP (B) 32 molecules of NADPH
(C) 30 molecules of ATP (D) 30 molecules of NADPH
Q.4 Ribulose-5-phosphate epimerase is involved in which one of the following processes?
(A) Glycolysis
(B) TCA cycle
(C) Glycosylation
(D) Pentose phosphate pathway
Q.5 Proteolytic enzymes are usually biosynthesized as large, inactive precursors known as
(A) holoenzymes (B) ribozyme
(C) zymogens (D) apoenzymes
Q.6 The formation of a carbocation, also called an oxonium ion, occurs during the reaction catalyzed by
(A) aldolase (B) lysozyme (C) ribonuclease A (D) ) carboxypeptidase
Q.7 Which one of the following amino acid substitutions is likely to cause the largest change in protein
conformation?
(A) Phe ? Ile (B) Ser ? Thr (C) Gln ? Tyr (D) Glu ? Val
Q.8 Which one of the following does NOT constitute the lipid moiety in lipid-linked membrane
proteins?
(A) Palmitic acid (B) Stearic acid
(C) Farnesyl groups (D) Myristic acid
Q.9 A closed circular B-DNA of 4000 base pairs is negatively supercoiled by introduction of 4 writhes.
The super helical density of the resultant DNA molecule will be _________
Q.10 Which one of the following is NOT a receptor tyrosine kinase?
(A) Platelet derived growth factor receptor
(B) Insulin like growth factor - 1 receptor
(C) Macrophage colony stimulating factor receptor
(D) Transforming growth factor ? receptor
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GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 1/5
I : BIOCHEMISTRY
Q. 1 ? Q. 10 carry one mark each.
Q.1 Which one of the following small molecules is a prerequisite for fatty acid oxidation?
(A) Inositol (B) Choline (C) Carnitine (D) Glycerol
Q.2 Which one of the following bases is NOT found in the T-arm of an aminoacyl t-RNA?
(A) Dihydrouridine
(B) Pseudouridine
(C) Uracil
(D) Guanine
Q.3 Oxidation of one molecule of glucose via the glycerol-phosphate shuttle produces
(A) 32 molecules of ATP (B) 32 molecules of NADPH
(C) 30 molecules of ATP (D) 30 molecules of NADPH
Q.4 Ribulose-5-phosphate epimerase is involved in which one of the following processes?
(A) Glycolysis
(B) TCA cycle
(C) Glycosylation
(D) Pentose phosphate pathway
Q.5 Proteolytic enzymes are usually biosynthesized as large, inactive precursors known as
(A) holoenzymes (B) ribozyme
(C) zymogens (D) apoenzymes
Q.6 The formation of a carbocation, also called an oxonium ion, occurs during the reaction catalyzed by
(A) aldolase (B) lysozyme (C) ribonuclease A (D) ) carboxypeptidase
Q.7 Which one of the following amino acid substitutions is likely to cause the largest change in protein
conformation?
(A) Phe ? Ile (B) Ser ? Thr (C) Gln ? Tyr (D) Glu ? Val
Q.8 Which one of the following does NOT constitute the lipid moiety in lipid-linked membrane
proteins?
(A) Palmitic acid (B) Stearic acid
(C) Farnesyl groups (D) Myristic acid
Q.9 A closed circular B-DNA of 4000 base pairs is negatively supercoiled by introduction of 4 writhes.
The super helical density of the resultant DNA molecule will be _________
Q.10 Which one of the following is NOT a receptor tyrosine kinase?
(A) Platelet derived growth factor receptor
(B) Insulin like growth factor - 1 receptor
(C) Macrophage colony stimulating factor receptor
(D) Transforming growth factor ? receptor
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 2/5
Q. 11 ? Q. 20 carry two marks each.
Q.11 Match the entries in Column-1 with those in Column-2
Column-1
(A) P-1; Q-4; R-2; S-6
Column-2
P. Vitamin B1 1. Thiamine pyrophosphate
Q. Carboxypeptidase 2. Aconitase
R. TCA cycle 3. Sucrose
S. Reducing sugar 4. Zn
2+
5. Riboflavin
6. Lactose
(B) P-5; Q-1; R-2; S-3
(C) P-1; Q-4; R-5; S-6
(D) P-5; Q-2; R-1; S-6
Q.12 The following table provides information about four proteins.
Which one of the following options correctly identifies the order of elution in size exclusion
chromatography and the increasing order of mobility in SDS polyacrylamide gel?
Protein Native mol. wt. (Da) pI Type
P 32000 6.4 monomer
Q 40000 8.5 homodimer
R 25000 4.9 monomer
S 45000 8.5 homotrimer
(A) Chromatrography: SQPR; Electrophoresis: RPQS
(B) Chromatrography: RPQS; Electrophoresis: SQPR
(C) Chromatrography: PRQS; Electrophoresis: PRQS
(D) Chromatrography: SQPR; Electrophoresis: PRQS
Q.13 The predicted molar extinction coefficient at 280 nm for the peptide
GEEFHISFLLIMFGAWSTHMYRTYWFIHEMISTRY is _______ M
-1
cm
-1
.
[Molar extinction coefficients for phenylalanine, tryptophan and tyrosine at 280 nm are 200, 5600
and 1400 M
-1
cm
-1
, respectively]
Q.14 Match the contents of Column I with the most appropriate options in Column II
Column I
(A) P-iii ; Q-v ; R-iv ; S-i
Column II
P. Complement C1q i. CD34
Q. L-Selectin ii. Complement C5b
R. Membrane Attack Complex iii. Fc region of antibody
S. T-Helper cells iv. Complement C5a
v. CD40L
(B) P-i ; Q-ii ; R-iv ; S-v
(C) P-iii ; Q-i ; R-ii ; S-v
(D) P-iv ; Q-v ; R-ii ; S-i
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GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 1/5
I : BIOCHEMISTRY
Q. 1 ? Q. 10 carry one mark each.
Q.1 Which one of the following small molecules is a prerequisite for fatty acid oxidation?
(A) Inositol (B) Choline (C) Carnitine (D) Glycerol
Q.2 Which one of the following bases is NOT found in the T-arm of an aminoacyl t-RNA?
(A) Dihydrouridine
(B) Pseudouridine
(C) Uracil
(D) Guanine
Q.3 Oxidation of one molecule of glucose via the glycerol-phosphate shuttle produces
(A) 32 molecules of ATP (B) 32 molecules of NADPH
(C) 30 molecules of ATP (D) 30 molecules of NADPH
Q.4 Ribulose-5-phosphate epimerase is involved in which one of the following processes?
(A) Glycolysis
(B) TCA cycle
(C) Glycosylation
(D) Pentose phosphate pathway
Q.5 Proteolytic enzymes are usually biosynthesized as large, inactive precursors known as
(A) holoenzymes (B) ribozyme
(C) zymogens (D) apoenzymes
Q.6 The formation of a carbocation, also called an oxonium ion, occurs during the reaction catalyzed by
(A) aldolase (B) lysozyme (C) ribonuclease A (D) ) carboxypeptidase
Q.7 Which one of the following amino acid substitutions is likely to cause the largest change in protein
conformation?
(A) Phe ? Ile (B) Ser ? Thr (C) Gln ? Tyr (D) Glu ? Val
Q.8 Which one of the following does NOT constitute the lipid moiety in lipid-linked membrane
proteins?
(A) Palmitic acid (B) Stearic acid
(C) Farnesyl groups (D) Myristic acid
Q.9 A closed circular B-DNA of 4000 base pairs is negatively supercoiled by introduction of 4 writhes.
The super helical density of the resultant DNA molecule will be _________
Q.10 Which one of the following is NOT a receptor tyrosine kinase?
(A) Platelet derived growth factor receptor
(B) Insulin like growth factor - 1 receptor
(C) Macrophage colony stimulating factor receptor
(D) Transforming growth factor ? receptor
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 2/5
Q. 11 ? Q. 20 carry two marks each.
Q.11 Match the entries in Column-1 with those in Column-2
Column-1
(A) P-1; Q-4; R-2; S-6
Column-2
P. Vitamin B1 1. Thiamine pyrophosphate
Q. Carboxypeptidase 2. Aconitase
R. TCA cycle 3. Sucrose
S. Reducing sugar 4. Zn
2+
5. Riboflavin
6. Lactose
(B) P-5; Q-1; R-2; S-3
(C) P-1; Q-4; R-5; S-6
(D) P-5; Q-2; R-1; S-6
Q.12 The following table provides information about four proteins.
Which one of the following options correctly identifies the order of elution in size exclusion
chromatography and the increasing order of mobility in SDS polyacrylamide gel?
Protein Native mol. wt. (Da) pI Type
P 32000 6.4 monomer
Q 40000 8.5 homodimer
R 25000 4.9 monomer
S 45000 8.5 homotrimer
(A) Chromatrography: SQPR; Electrophoresis: RPQS
(B) Chromatrography: RPQS; Electrophoresis: SQPR
(C) Chromatrography: PRQS; Electrophoresis: PRQS
(D) Chromatrography: SQPR; Electrophoresis: PRQS
Q.13 The predicted molar extinction coefficient at 280 nm for the peptide
GEEFHISFLLIMFGAWSTHMYRTYWFIHEMISTRY is _______ M
-1
cm
-1
.
[Molar extinction coefficients for phenylalanine, tryptophan and tyrosine at 280 nm are 200, 5600
and 1400 M
-1
cm
-1
, respectively]
Q.14 Match the contents of Column I with the most appropriate options in Column II
Column I
(A) P-iii ; Q-v ; R-iv ; S-i
Column II
P. Complement C1q i. CD34
Q. L-Selectin ii. Complement C5b
R. Membrane Attack Complex iii. Fc region of antibody
S. T-Helper cells iv. Complement C5a
v. CD40L
(B) P-i ; Q-ii ; R-iv ; S-v
(C) P-iii ; Q-i ; R-ii ; S-v
(D) P-iv ; Q-v ; R-ii ; S-i
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 3/5
Q.15
The value of ?G at 37 ?C for the movement of Ca
2+
ions from the endoplasmic reticulum where
[Ca
2+
] is 1 mM to the cytosol where [Ca
2+
] is 0.1 ?M at ?50 mV membrane potential is
_______________ kJ mol
-1
.
[ R = 8.314 JK
?1
mol
?1
and 1 Faraday = 96500 Coulombs]
Q.16
C
?
N
C
?
N
C
?
R
1
C
O
O
C
H
R
2
H
R
3
W
X Y
Z
Column I Column II
W i. ?
X ii. ?
Y iii. ?
Z iv. ?
Which of the following identifies the correctly matched pairs?
(A) W-iii ; X-i ; Y-iv ; Z-ii
(B) W-i ; X-iii ; Y-iv ; Z-ii
(C) W-i ; X-iii ; Y-ii ; Z-iv
(D) W-iii ; X-i ; Y-ii ; Z-iv
Q.17 Which of the following statements is/are INCORRECT about hemoglobin (Hb)?
I. Hb demonstrates higher oxygen carrying capacity compared to myoglobin
II. There is covalent bonding between the four subunits of Hb
III. During deoxygenation the loss of the first oxygen molecule from oxygenated Hb promotes
the dissociation of oxygen from the other subunits
(A) II (B) II & III (C) I & III (D) III
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GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 1/5
I : BIOCHEMISTRY
Q. 1 ? Q. 10 carry one mark each.
Q.1 Which one of the following small molecules is a prerequisite for fatty acid oxidation?
(A) Inositol (B) Choline (C) Carnitine (D) Glycerol
Q.2 Which one of the following bases is NOT found in the T-arm of an aminoacyl t-RNA?
(A) Dihydrouridine
(B) Pseudouridine
(C) Uracil
(D) Guanine
Q.3 Oxidation of one molecule of glucose via the glycerol-phosphate shuttle produces
(A) 32 molecules of ATP (B) 32 molecules of NADPH
(C) 30 molecules of ATP (D) 30 molecules of NADPH
Q.4 Ribulose-5-phosphate epimerase is involved in which one of the following processes?
(A) Glycolysis
(B) TCA cycle
(C) Glycosylation
(D) Pentose phosphate pathway
Q.5 Proteolytic enzymes are usually biosynthesized as large, inactive precursors known as
(A) holoenzymes (B) ribozyme
(C) zymogens (D) apoenzymes
Q.6 The formation of a carbocation, also called an oxonium ion, occurs during the reaction catalyzed by
(A) aldolase (B) lysozyme (C) ribonuclease A (D) ) carboxypeptidase
Q.7 Which one of the following amino acid substitutions is likely to cause the largest change in protein
conformation?
(A) Phe ? Ile (B) Ser ? Thr (C) Gln ? Tyr (D) Glu ? Val
Q.8 Which one of the following does NOT constitute the lipid moiety in lipid-linked membrane
proteins?
(A) Palmitic acid (B) Stearic acid
(C) Farnesyl groups (D) Myristic acid
Q.9 A closed circular B-DNA of 4000 base pairs is negatively supercoiled by introduction of 4 writhes.
The super helical density of the resultant DNA molecule will be _________
Q.10 Which one of the following is NOT a receptor tyrosine kinase?
(A) Platelet derived growth factor receptor
(B) Insulin like growth factor - 1 receptor
(C) Macrophage colony stimulating factor receptor
(D) Transforming growth factor ? receptor
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 2/5
Q. 11 ? Q. 20 carry two marks each.
Q.11 Match the entries in Column-1 with those in Column-2
Column-1
(A) P-1; Q-4; R-2; S-6
Column-2
P. Vitamin B1 1. Thiamine pyrophosphate
Q. Carboxypeptidase 2. Aconitase
R. TCA cycle 3. Sucrose
S. Reducing sugar 4. Zn
2+
5. Riboflavin
6. Lactose
(B) P-5; Q-1; R-2; S-3
(C) P-1; Q-4; R-5; S-6
(D) P-5; Q-2; R-1; S-6
Q.12 The following table provides information about four proteins.
Which one of the following options correctly identifies the order of elution in size exclusion
chromatography and the increasing order of mobility in SDS polyacrylamide gel?
Protein Native mol. wt. (Da) pI Type
P 32000 6.4 monomer
Q 40000 8.5 homodimer
R 25000 4.9 monomer
S 45000 8.5 homotrimer
(A) Chromatrography: SQPR; Electrophoresis: RPQS
(B) Chromatrography: RPQS; Electrophoresis: SQPR
(C) Chromatrography: PRQS; Electrophoresis: PRQS
(D) Chromatrography: SQPR; Electrophoresis: PRQS
Q.13 The predicted molar extinction coefficient at 280 nm for the peptide
GEEFHISFLLIMFGAWSTHMYRTYWFIHEMISTRY is _______ M
-1
cm
-1
.
[Molar extinction coefficients for phenylalanine, tryptophan and tyrosine at 280 nm are 200, 5600
and 1400 M
-1
cm
-1
, respectively]
Q.14 Match the contents of Column I with the most appropriate options in Column II
Column I
(A) P-iii ; Q-v ; R-iv ; S-i
Column II
P. Complement C1q i. CD34
Q. L-Selectin ii. Complement C5b
R. Membrane Attack Complex iii. Fc region of antibody
S. T-Helper cells iv. Complement C5a
v. CD40L
(B) P-i ; Q-ii ; R-iv ; S-v
(C) P-iii ; Q-i ; R-ii ; S-v
(D) P-iv ; Q-v ; R-ii ; S-i
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 3/5
Q.15
The value of ?G at 37 ?C for the movement of Ca
2+
ions from the endoplasmic reticulum where
[Ca
2+
] is 1 mM to the cytosol where [Ca
2+
] is 0.1 ?M at ?50 mV membrane potential is
_______________ kJ mol
-1
.
[ R = 8.314 JK
?1
mol
?1
and 1 Faraday = 96500 Coulombs]
Q.16
C
?
N
C
?
N
C
?
R
1
C
O
O
C
H
R
2
H
R
3
W
X Y
Z
Column I Column II
W i. ?
X ii. ?
Y iii. ?
Z iv. ?
Which of the following identifies the correctly matched pairs?
(A) W-iii ; X-i ; Y-iv ; Z-ii
(B) W-i ; X-iii ; Y-iv ; Z-ii
(C) W-i ; X-iii ; Y-ii ; Z-iv
(D) W-iii ; X-i ; Y-ii ; Z-iv
Q.17 Which of the following statements is/are INCORRECT about hemoglobin (Hb)?
I. Hb demonstrates higher oxygen carrying capacity compared to myoglobin
II. There is covalent bonding between the four subunits of Hb
III. During deoxygenation the loss of the first oxygen molecule from oxygenated Hb promotes
the dissociation of oxygen from the other subunits
(A) II (B) II & III (C) I & III (D) III
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 4/5
Q.18 A 1.2 kb DNA fragment was used as a template for PCR amplification using primers P1, P2, P3
and P4 as shown in the scheme below. The annealing positions of primers on the template are
indicated by numbers. Primers P2 and P3 contain single base mismatches as indicated by filled
triangles.
PCR was performed using primer pair P1 and P3 in one vial and P2 and P4 in another vial. The
purified PCR products from the two vials were mixed and subjected to another round of PCR with
primers P1 and P4. The final PCR product will correspond to a
(A) 1.2 kb wild type DNA
(B) 1.2 kb DNA with two point mutations
(C) 0.9 kb DNA with one point mutation
(D) 0.5 kb DNA with one point mutation
Q.19 A cell suspension was subjected to membrane disruption followed by differential centrifugation to
fractionate the cellular components.
Match the centrifugal conditions in Column I to the appropriate subcellular components in Column
II.
Column I
(A) P-iii ; Q-iv ; R-i ; S-ii
Column II
P. 1000 g, 10 min i. Microsomes and small vesicles
Q. 20000 g, 30 min ii. Ribosomes
R. 80000 g, 1 hour iii. Nuclei
S. 150000 g, 3 hours iv. Lysosomes and peroxisomes
(B) P-i ; Q-iv ; R-iii ; S-ii
(C) P-iii ; Q-iv ; R-ii ; S-i
(D) P-ii ; Q-i ; R-iv ; S-iii
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GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 1/5
I : BIOCHEMISTRY
Q. 1 ? Q. 10 carry one mark each.
Q.1 Which one of the following small molecules is a prerequisite for fatty acid oxidation?
(A) Inositol (B) Choline (C) Carnitine (D) Glycerol
Q.2 Which one of the following bases is NOT found in the T-arm of an aminoacyl t-RNA?
(A) Dihydrouridine
(B) Pseudouridine
(C) Uracil
(D) Guanine
Q.3 Oxidation of one molecule of glucose via the glycerol-phosphate shuttle produces
(A) 32 molecules of ATP (B) 32 molecules of NADPH
(C) 30 molecules of ATP (D) 30 molecules of NADPH
Q.4 Ribulose-5-phosphate epimerase is involved in which one of the following processes?
(A) Glycolysis
(B) TCA cycle
(C) Glycosylation
(D) Pentose phosphate pathway
Q.5 Proteolytic enzymes are usually biosynthesized as large, inactive precursors known as
(A) holoenzymes (B) ribozyme
(C) zymogens (D) apoenzymes
Q.6 The formation of a carbocation, also called an oxonium ion, occurs during the reaction catalyzed by
(A) aldolase (B) lysozyme (C) ribonuclease A (D) ) carboxypeptidase
Q.7 Which one of the following amino acid substitutions is likely to cause the largest change in protein
conformation?
(A) Phe ? Ile (B) Ser ? Thr (C) Gln ? Tyr (D) Glu ? Val
Q.8 Which one of the following does NOT constitute the lipid moiety in lipid-linked membrane
proteins?
(A) Palmitic acid (B) Stearic acid
(C) Farnesyl groups (D) Myristic acid
Q.9 A closed circular B-DNA of 4000 base pairs is negatively supercoiled by introduction of 4 writhes.
The super helical density of the resultant DNA molecule will be _________
Q.10 Which one of the following is NOT a receptor tyrosine kinase?
(A) Platelet derived growth factor receptor
(B) Insulin like growth factor - 1 receptor
(C) Macrophage colony stimulating factor receptor
(D) Transforming growth factor ? receptor
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 2/5
Q. 11 ? Q. 20 carry two marks each.
Q.11 Match the entries in Column-1 with those in Column-2
Column-1
(A) P-1; Q-4; R-2; S-6
Column-2
P. Vitamin B1 1. Thiamine pyrophosphate
Q. Carboxypeptidase 2. Aconitase
R. TCA cycle 3. Sucrose
S. Reducing sugar 4. Zn
2+
5. Riboflavin
6. Lactose
(B) P-5; Q-1; R-2; S-3
(C) P-1; Q-4; R-5; S-6
(D) P-5; Q-2; R-1; S-6
Q.12 The following table provides information about four proteins.
Which one of the following options correctly identifies the order of elution in size exclusion
chromatography and the increasing order of mobility in SDS polyacrylamide gel?
Protein Native mol. wt. (Da) pI Type
P 32000 6.4 monomer
Q 40000 8.5 homodimer
R 25000 4.9 monomer
S 45000 8.5 homotrimer
(A) Chromatrography: SQPR; Electrophoresis: RPQS
(B) Chromatrography: RPQS; Electrophoresis: SQPR
(C) Chromatrography: PRQS; Electrophoresis: PRQS
(D) Chromatrography: SQPR; Electrophoresis: PRQS
Q.13 The predicted molar extinction coefficient at 280 nm for the peptide
GEEFHISFLLIMFGAWSTHMYRTYWFIHEMISTRY is _______ M
-1
cm
-1
.
[Molar extinction coefficients for phenylalanine, tryptophan and tyrosine at 280 nm are 200, 5600
and 1400 M
-1
cm
-1
, respectively]
Q.14 Match the contents of Column I with the most appropriate options in Column II
Column I
(A) P-iii ; Q-v ; R-iv ; S-i
Column II
P. Complement C1q i. CD34
Q. L-Selectin ii. Complement C5b
R. Membrane Attack Complex iii. Fc region of antibody
S. T-Helper cells iv. Complement C5a
v. CD40L
(B) P-i ; Q-ii ; R-iv ; S-v
(C) P-iii ; Q-i ; R-ii ; S-v
(D) P-iv ; Q-v ; R-ii ; S-i
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 3/5
Q.15
The value of ?G at 37 ?C for the movement of Ca
2+
ions from the endoplasmic reticulum where
[Ca
2+
] is 1 mM to the cytosol where [Ca
2+
] is 0.1 ?M at ?50 mV membrane potential is
_______________ kJ mol
-1
.
[ R = 8.314 JK
?1
mol
?1
and 1 Faraday = 96500 Coulombs]
Q.16
C
?
N
C
?
N
C
?
R
1
C
O
O
C
H
R
2
H
R
3
W
X Y
Z
Column I Column II
W i. ?
X ii. ?
Y iii. ?
Z iv. ?
Which of the following identifies the correctly matched pairs?
(A) W-iii ; X-i ; Y-iv ; Z-ii
(B) W-i ; X-iii ; Y-iv ; Z-ii
(C) W-i ; X-iii ; Y-ii ; Z-iv
(D) W-iii ; X-i ; Y-ii ; Z-iv
Q.17 Which of the following statements is/are INCORRECT about hemoglobin (Hb)?
I. Hb demonstrates higher oxygen carrying capacity compared to myoglobin
II. There is covalent bonding between the four subunits of Hb
III. During deoxygenation the loss of the first oxygen molecule from oxygenated Hb promotes
the dissociation of oxygen from the other subunits
(A) II (B) II & III (C) I & III (D) III
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 4/5
Q.18 A 1.2 kb DNA fragment was used as a template for PCR amplification using primers P1, P2, P3
and P4 as shown in the scheme below. The annealing positions of primers on the template are
indicated by numbers. Primers P2 and P3 contain single base mismatches as indicated by filled
triangles.
PCR was performed using primer pair P1 and P3 in one vial and P2 and P4 in another vial. The
purified PCR products from the two vials were mixed and subjected to another round of PCR with
primers P1 and P4. The final PCR product will correspond to a
(A) 1.2 kb wild type DNA
(B) 1.2 kb DNA with two point mutations
(C) 0.9 kb DNA with one point mutation
(D) 0.5 kb DNA with one point mutation
Q.19 A cell suspension was subjected to membrane disruption followed by differential centrifugation to
fractionate the cellular components.
Match the centrifugal conditions in Column I to the appropriate subcellular components in Column
II.
Column I
(A) P-iii ; Q-iv ; R-i ; S-ii
Column II
P. 1000 g, 10 min i. Microsomes and small vesicles
Q. 20000 g, 30 min ii. Ribosomes
R. 80000 g, 1 hour iii. Nuclei
S. 150000 g, 3 hours iv. Lysosomes and peroxisomes
(B) P-i ; Q-iv ; R-iii ; S-ii
(C) P-iii ; Q-iv ; R-ii ; S-i
(D) P-ii ; Q-i ; R-iv ; S-iii
GATE 2015 BIOCHEMISTRY ? XL-I
XL-I 5/5
Q.20 Given below are the maps of a 1200 base pairs (bp) long DNA insert and a 3000 bp expression
vector. The BamHI (B) and HindIII (H) restriction sites and DNA length between them are
indicated in base pairs.
The insert is cloned into the vector at the BamHI site and the desired orientation is shown by the
arrow. After cloning, the orientation of the insert in the recombinant plasmid is tested by complete
HindIII digestion followed by agarose gel electrophoresis. Which one of the following band
patterns reveals the correct orientation of the insert in the construct?
(A)
(B)
(C)
(D)
END OF THE QUESTION PAPER
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This post was last modified on 19 December 2019