Download GTU BE/B.Tech 2019 Winter 5th Sem New 2150401 Advanced Molecular Biology I Question Paper

Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2019 Winter 5th Sem New 2150401 Advanced Molecular Biology I Previous Question Paper

1
Seat No.: ________ Enrolment No.___________

GUJARAT TECHNOLOGICAL UNIVERSITY

BE - SEMESTER ? V (New) EXAMINATION ? WINTER 2019
Subject Code: 2150401 Date: 25/11/2019

Subject Name: Advanced Molecular Biology - I
Time: 10:30 AM TO 01:00 PM Total Marks: 70

Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

MARKS

Q.1 (a) Give functions of: DNA Ligase, Restriction Endonuclease, Linker
sequence.
03
(b) Explain steps of gene cloning in brief. 04
(c) Explain the technique of Southern Hybridization. 07

Q.2 (a) Compare sticky and blunt ends generated by RE. 03
(b) Explain structure and regulation of Fertility plasmid. 04
(c) Explain any one vector in detail along with its markers. 07
OR
(c) Explain the possiblities for insertion of DNA insert into vector. 07
Q.3 (a) What are clonning and expression vectors? 03
(b) Explain the process of formation of holliday junction. 04
(c) Explain principle and working of PCR. 07
OR
Q.3 (a) Explain principle of electrophoresis. 03
(b) How F is prime generated in E.coli? 04
(c) Explain double strand break model in recombination. 07
Q.4 (a) Compare cDNA and Genomic library. 03
(b) What are the methods to induce artificial competence? 04
(c) Explain process of transfer of DNA from donor to recipient during
conjugation process.
07
OR
Q.4 (a) What are Hfr Strains? 03
(b) What is transduction? 04
(c) Explain the process of development of natural competence in
Streptococcus.
07
Q.5 (a) Give properties of good host. 03
(b) Explain concept of reverse genetics. 04
(c) Explain abortive transduction in detail. 07
OR

Q.5 (a) Compare DNA transfer in conjugation, transduction and
transformation.
03
(b) Narrate applications of gene therapy. 04
(c) Explain specialized transduction in detail. 07

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