Download GTU BE/B.Tech 2019 Winter 6th Sem New 2160401 Advanced Molecular Biology Ii Question Paper

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GUJARAT TECHNOLOGICAL UNIVERSITY

BE - SEMESTER- VI (New) EXAMINATION - WINTER 2019

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Subject Code: 2160401

Subject Name: Advanced Molecular Biology-II

Time: 02:30 PM TO 05:00 PM

Date: 04/12/2019

Total Marks: 70

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Instructions:

1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1

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1. What do you mean by mapping of genes? What is the significance of gene mapping? [03]
2. Explain trans splicing and alternative splicing. [04]
3. Describe the mechanism of lariat formation during splicing process. [07]

Q.2

1. How do intercalating agents lead to mutations? [03]

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2. Compare missense and non sense mutations. [04]
3. Explain base substitution mutation and its effects with proper illustration. [07]

OR

1. Explain frameshift mutation and its effects with proper illustration [07]

Q.3

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1. Draw a neat diagram of retrovirus. [03]
2. Compare lytic and lysogenic life cycles of virus. [04]
3. Explain intragenic suppression of mutation with proper example. [07]

OR

1. What is primer walking? [03]

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2. What is gene replacement and gene targeting? [04]
3. Explain effect of Ultraviolet radiation on DNA. [07]

Q.4

1. What are transposable elements? Give its types. [03]
2. Explain structure of trp operon. [04]

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3. Explain the process of assembly and maturation of T phage in its host. [07]

OR

1. What is catabolite repression? [03]
2. Explain principle of LM PCR. [04]
3. Explain Base Excision Repair in detail. [07]

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Q.5

1. What are self splicing RNAs? [03]
2. Enlist different methods for regulation of metabolic pathway. Explain any one in detail. [04]
3. Explain principle, mechanism and application of DNA Fingerprinting technique. [07]

OR

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1. What is linkage disequilibrium? [03]
2. Write a note on retrotransposon. [04]
3. Explain positive regulation of lac operon. [07]

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