GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER- VI (New) EXAMINATION - WINTER 2019
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Subject Code: 2160508 Date: 11/12/2019Subject Name: Biochemical Engineering
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
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2. Make suitable assumptions wherever necessary.3. Figures to the right indicate full marks.
MARKS | ||
Q.1 (a) | List various unit operation encountered in bioprocessing operation. | 03 |
(b) | What are the various methods used for immobilization of enzymes. | 04 |
(c) | Write in brief notes on various steps involved in integrated bioprocessing. | 07 |
Q.2 (a) | How does internal feedback system work for biomass? | 03 |
(b) | Explain with a diagram different phases of microbial growth. | 04 |
(c) | Explain growth of a typical microbial culture in a batch conditions. | 07 |
OR | ||
(c) | Discuss the relative advantages and disadvantages of continuous culture. | 07 |
Q.3 (a) | Explain the following valve: (1) Plug valve (2) Butterfly valve (3) Ball Valve. | 03 |
(b) | Write in brief the air sterilization technique used for aerobic fermentation process. | 04 |
(c) | Draw a schematic of a fermenter vessel. Label the major components and briefly explain their functions. | 07 |
OR | ||
Q.3 (a) | State various strategies of aeration in fermentation vessel. | 03 |
(b) | Enlist various process and monitoring parameters for a control of a fermenter. | 04 |
(c) | Discuss the different types of spargers used for aeration in fermenter. | 07 |
Q.4 (a) | Define fluid rheology and its importance in fermentation process. | 03 |
(b) | Explain in short oxygen supply in fermentation process. | 04 |
(c) | List out various methods for determination of KLa value. Explain any one in detail. | 07 |
OR | ||
Q.4 (a) | Explain briefly the principle of scale up in bioprocess operation. | 03 |
(b) | Explain the effect of various physico chemical parameter on Ka. | 04 |
A Newtonian broth in the very initial stage of fermentation.
(1) Find out whether the fluid is Newtonian or non-Newtonian. If it is non- Write in short on drying and crystallization process for separation and purification of product Newtonian fluid, find the rheological constants.
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(1) If it is a Newtonian fluid, find the viscosity of the fluid.t, N/m² | ?, S! |
---|---|
100 | 5 |
210 | 10 |
295 | 15 |
410 | 20 |
620 | 32 |
1000 | 50 |
Q.5 (a) | Write in short on drying and crystallization process for separation and purification of products. | 03 |
(b) | Write a short note on crystallizer. | 04 |
(c) | What is the purpose of cell disruption? Discuss various methods of cell disruption briefly. | 07 |
OR | ||
Q.5 (a) | Explain range of fermentation process. | 03 |
(b) | Explain different types of membrane process and their specification. | 04 |
(c) | Explain single stage liquid-liquid extraction. | 07 |
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