Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2019 Winter 3rd Sem New 2131407 Basic Food Microbiology Previous Question Paper
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ? III (New) EXAMINATION ? WINTER 2019
Subject Code: 2131407 Date: 30/11/2019
Subject Name: Basic Food Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom classification
based on characteristics.
03
(b) Describe koch postulates in form of flowchart. 04
(c) Describe the difference between gram +ve and gram ?ve bacteria.
Give example of gram +ve rod, gram +ve cocci, gram ?ve rod, gram
?ve cocci
07
Q.2 (a) Draw a diagram to depict procedure of gram staining 03
(b) Draw a flowchart to depict the health benefits rendered by probiotics.
Give examples for the probiotics which render health benefit in large
intestine and small intestine
04
(c) Draw flowchart to depict the steps of western blotting technique and
suggest its applications
07
OR
(c) Describe the difference between prokaryote and eukaryote along
with diagram
07
Q.3 (a) Bacteria are farmers best friends but sometimes may also act as
enemy. Explain the statement with examples.
03
(b) Write a short note on chemical and physical methods to control
microorganisms
04
(c) Louis Pasteur has contributed towards significant milestones in
microbiology. Justify the statement with examples
07
OR
Q.3 (a) Give example of bacteria of following categories a) photoautotrophic
b) photoheterotroph c) chemoautotroph
03
(b) Explain replica plating for the selection of auxotrophc strains. How
do auxotrophic strain differ from bradytrophic strain
04
(c) Describe characteristics of each phase depicted in the given diagram.
Calculate the doubling time from the given graph. Derive the
microbial generation time equation.
07
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Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ? III (New) EXAMINATION ? WINTER 2019
Subject Code: 2131407 Date: 30/11/2019
Subject Name: Basic Food Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom classification
based on characteristics.
03
(b) Describe koch postulates in form of flowchart. 04
(c) Describe the difference between gram +ve and gram ?ve bacteria.
Give example of gram +ve rod, gram +ve cocci, gram ?ve rod, gram
?ve cocci
07
Q.2 (a) Draw a diagram to depict procedure of gram staining 03
(b) Draw a flowchart to depict the health benefits rendered by probiotics.
Give examples for the probiotics which render health benefit in large
intestine and small intestine
04
(c) Draw flowchart to depict the steps of western blotting technique and
suggest its applications
07
OR
(c) Describe the difference between prokaryote and eukaryote along
with diagram
07
Q.3 (a) Bacteria are farmers best friends but sometimes may also act as
enemy. Explain the statement with examples.
03
(b) Write a short note on chemical and physical methods to control
microorganisms
04
(c) Louis Pasteur has contributed towards significant milestones in
microbiology. Justify the statement with examples
07
OR
Q.3 (a) Give example of bacteria of following categories a) photoautotrophic
b) photoheterotroph c) chemoautotroph
03
(b) Explain replica plating for the selection of auxotrophc strains. How
do auxotrophic strain differ from bradytrophic strain
04
(c) Describe characteristics of each phase depicted in the given diagram.
Calculate the doubling time from the given graph. Derive the
microbial generation time equation.
07
2
Q.4 (a) What are intrinsic and extrinsic factors, which affect the microbial
load?
03
(b) Explain the concept of each test included in IMViC test. Suggest an
application of the same.
04
(c) An apple pie sample contained 10000 cells of S. aureus. And it was
kept at room temperature for 4 hrs. What would be the generation
time if the final no. of cells is 6.5 X 10^8.
07
OR
Q.4 (a) Explain the formation of HFr and F? strains formed during bacterial
conjugation. Also write about the significance of HFr and F?
03
(b) Draw a flowchart to explain experiment which proved the bacterial
transformation. Write the name of scientist who conducted the
experiment, strain of bacteria used, scientist/s who proved the
transforming principle.
04
(c) Draw a diagram to depict a typical PCR cycle and detection of PCR
product. Enlist the ingredients in PCR reaction. What is the
significance of Taq polymerase? Which enzymes are actually used
by cells for removing primers, and filling in gap, joining the strands
07
Q.5 (a) Write a short note on contribution of Lady Mary Wortley Montagu
in the field of immunology
03
(b) What is holliday junction? Draw the steps for the same. 04
(c) Draw flowchart depicting steps of ELISA. What is sandwich ELISA?
Suggest few applications, advantages and disadvantages of ELISA.
Explain how it is semiquantitative assay.
07
OR
Q.5 (a) If 220 colonies were obtained on a given plate which was prepared
by pour plating with 7th dilution, determine the microbial count in
terms of log cfu/ml
03
(b) Draw a flowchart to depict long term preservation of cultures at
10?C, -20?C, -80?C and -196?C
04
(c) Draw steps of bacterial transduction. Comment on prophage,
generalized transduction and specialized transduction.
07
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This post was last modified on 20 February 2020