Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2019 Summer 6th Sem New 2160508 Biochemical Engineering Previous Question Paper
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?VI(NEW) ? EXAMINATION ? SUMMER 2019
Subject Code:2160508 Date:18/05/2019
Subject Name:Biochemical Engineering
Time:10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Understand the question carefully and write in proportion to marks it carries.
3. Provide explanation wherever required.
4. Figures to the right indicate full marks.
Q.1 (a) State three differences between a chemical reaction and its
biochemical counterpart.
03
(b) Explain with a diagram various phases of microbial growth. Which
phase is the longest?
04
(c) Discuss with examples the application of fermentation technology in
food and beverage, industrial solvents, and antibiotics production.
07
Q.2 (a) Name three industrially important biochemical reactions and mention
the name(s) of the microorganism involved therein.
03
(b) State and explain different types of solid and liquid media used for
microbial growth.
04
(c) Discuss various methods of cell disruption to get intracellular
products. Give two examples of such intracellular products.
07
OR
(c) State and briefly explain various steps, either sequential or
concurrent, of an integrated bioprocess in general.
07
Q.3 (a) State three methods for the measurement of microbial growth. 03
(b) Write down Monod equation of microbial growth kinetics. Explain
various terms in the same equation. How do you determine the kinetic
parameters of the above equation graphically?
04
(c) Using substrate and biomass balance derive the design equation for a
continuous stirred tank system for biochemical process. State the
assumptions. Comment on critical dilution rate and washout factor.
07
OR
Q.3 (a) What are the temperature, pressure and time conditions for steam
sterilization? Name a few methods for sterilization of medium other
than using steam.
03
(b) Calculate the steady state substrate and biomass concentration in a
continuous fermenter which has an operating volume of 25 L. When
the sterile feed stream contains limiting substrate at 2000 mg/L and
enters the vessel at 8L/h. The values of Ks and ?m are 10.5 mg/L and
0.45 h
?1
respectively and the yield coefficient may be taken as 0.48.
04
(c) Illustrate various probable configurations of fed-batch bioreactors
with schematic diagrams.
07
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GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?VI(NEW) ? EXAMINATION ? SUMMER 2019
Subject Code:2160508 Date:18/05/2019
Subject Name:Biochemical Engineering
Time:10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Understand the question carefully and write in proportion to marks it carries.
3. Provide explanation wherever required.
4. Figures to the right indicate full marks.
Q.1 (a) State three differences between a chemical reaction and its
biochemical counterpart.
03
(b) Explain with a diagram various phases of microbial growth. Which
phase is the longest?
04
(c) Discuss with examples the application of fermentation technology in
food and beverage, industrial solvents, and antibiotics production.
07
Q.2 (a) Name three industrially important biochemical reactions and mention
the name(s) of the microorganism involved therein.
03
(b) State and explain different types of solid and liquid media used for
microbial growth.
04
(c) Discuss various methods of cell disruption to get intracellular
products. Give two examples of such intracellular products.
07
OR
(c) State and briefly explain various steps, either sequential or
concurrent, of an integrated bioprocess in general.
07
Q.3 (a) State three methods for the measurement of microbial growth. 03
(b) Write down Monod equation of microbial growth kinetics. Explain
various terms in the same equation. How do you determine the kinetic
parameters of the above equation graphically?
04
(c) Using substrate and biomass balance derive the design equation for a
continuous stirred tank system for biochemical process. State the
assumptions. Comment on critical dilution rate and washout factor.
07
OR
Q.3 (a) What are the temperature, pressure and time conditions for steam
sterilization? Name a few methods for sterilization of medium other
than using steam.
03
(b) Calculate the steady state substrate and biomass concentration in a
continuous fermenter which has an operating volume of 25 L. When
the sterile feed stream contains limiting substrate at 2000 mg/L and
enters the vessel at 8L/h. The values of Ks and ?m are 10.5 mg/L and
0.45 h
?1
respectively and the yield coefficient may be taken as 0.48.
04
(c) Illustrate various probable configurations of fed-batch bioreactors
with schematic diagrams.
07
Q.4 (a) Write down the differences between prokaryotic and eukaryotic cells
with examples.
03
(b) How does an enzyme work? Classify enzymes with examples. 04
(c) Draw a schematic diagram of a fermenter, label its different parts and
state the functions of major parts.
07
OR
Q.4. (a) What are different types of controlling system in fermenter? 03
(b) Write a note on biosensors for fermentation control. 04
(c) Discuss various monitoring and control parameters of an ideal
fermenter.
07
Q.5 (a) Explain why oxygen needs to be supplied at a sufficient rate during
aerobic fermentation.
03
(b) Explain various physico-chemical parameters affecting volumetric
mass transfer coefficient KLa.
04
(c) State various methods of determination of volumetric mass transfer
coefficient KLa in a fermenter and explain any one.
07
OR
Q.5 (a) Name various unit operations used in upstream and down stream
processing in fermentation.
03
(b) Discuss briefly the rheological behaviour of fermentation broth with
appropriate examples.
04
(c) Explain differential centrifugation method for separation of cells
from fermentation broth.
07
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This post was last modified on 20 February 2020