Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2019 Summer 3rd Sem New 2131407 Basic Food Microbiology Previous Question Paper
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?III (NEW) EXAMINATION ? SUMMER 2019
Subject Code: 2131407 Date: 07/06/2019
Subject Name:Basic Food Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Draw diagrams wherever necessary.
5. Write scientific names as per the norms
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom classification based on
characteristics.
03
(b) Justify why Robert Koch is also known as Father of Modern bacteriology 04
(c) Describe the difference between gram +ve and gram ?ve bacteria. Give
example of gram +ve rod, gram +ve cocci, gram ?ve rod, gram ?ve cocci
07
Q.2 (a) Write a short note on chemical and physical methods to control microorganisms 03
(b) Microbial growth occurs is observed as sigmoidal curve. Describe each phase
of the sigmoidal curve. During which phase the primary metabolites are
formed?
04
(c) Describe the difference between eukaryotic and prokaryotic cell 07
OR
(c) Justify he statement ?Louis Pasteur is aptly known as Father of Microbiology 07
Q.3 (a) An apple pie sample contained 10000 cells of S. aureus. And it was kept at
room temperature for 4 hrs. What would be the generation time if the final no.
of cells is 6.5 X 10^8.
03
(b) Explain Redi?s, Needham?s and Spallanzani?s experiments. Which scientist
favored concept of biogenesis
04
(c) Using a schematic diagram, describe the steps of western blotting technique.
For which substance, northern blotting is used?
07
OR
Q.3 (a) Draw a diagram to depict procedure of gram staining 03
(b) Draw a flowchart to depict the health benefits rendered by probiotics. Give
examples for the probiotics which render health benefit in large intestine and
small intestine
04
(c) Describe PCR, its applications, advantages and disadvantages. Draw a diagram
to depict PCR cycle and enlist the ingredients used for PCR
07
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GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?III (NEW) EXAMINATION ? SUMMER 2019
Subject Code: 2131407 Date: 07/06/2019
Subject Name:Basic Food Microbiology
Time: 02:30 PM TO 05:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Draw diagrams wherever necessary.
5. Write scientific names as per the norms
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom classification based on
characteristics.
03
(b) Justify why Robert Koch is also known as Father of Modern bacteriology 04
(c) Describe the difference between gram +ve and gram ?ve bacteria. Give
example of gram +ve rod, gram +ve cocci, gram ?ve rod, gram ?ve cocci
07
Q.2 (a) Write a short note on chemical and physical methods to control microorganisms 03
(b) Microbial growth occurs is observed as sigmoidal curve. Describe each phase
of the sigmoidal curve. During which phase the primary metabolites are
formed?
04
(c) Describe the difference between eukaryotic and prokaryotic cell 07
OR
(c) Justify he statement ?Louis Pasteur is aptly known as Father of Microbiology 07
Q.3 (a) An apple pie sample contained 10000 cells of S. aureus. And it was kept at
room temperature for 4 hrs. What would be the generation time if the final no.
of cells is 6.5 X 10^8.
03
(b) Explain Redi?s, Needham?s and Spallanzani?s experiments. Which scientist
favored concept of biogenesis
04
(c) Using a schematic diagram, describe the steps of western blotting technique.
For which substance, northern blotting is used?
07
OR
Q.3 (a) Draw a diagram to depict procedure of gram staining 03
(b) Draw a flowchart to depict the health benefits rendered by probiotics. Give
examples for the probiotics which render health benefit in large intestine and
small intestine
04
(c) Describe PCR, its applications, advantages and disadvantages. Draw a diagram
to depict PCR cycle and enlist the ingredients used for PCR
07
Q.4 (a) Describe steps of bacterial DNA exchange by conjugation using a well labeled
diagram. Also write about the significance of HFr and F?
03
(b) Describe morphology of bacteria and conventions followed for nomenclature
of microorganisms
04
(c) Bacteria change from non-pathogenic strain to pathogenic strain. Explain the
concept with experiment conducted by Griffith. Also suggest an application in
reference to food industry.
07
OR
Q.4 (a) Draw a diagram to depict parts of compound bright field microscope. Why
immersion oil is necessary while using 100X objective?
03
(b) Draw a diagram to depict Holliday junction. How does it helps in evolution? 04
(c) Draw A diagram to depict lysogenic and lytic phases of transduction. Explain
the concept of Specialized transduction.
07
Q.5 (a) What is direct and indirect ELISA and list down applications. 03
(b) Write a short note on contribution of Lady Mary Wortley Montagu in the field
of immunology
04
(c) Explain the concept of IMViC test used for differentiating enteric bacteria 07
OR
Q.5 (a) If 220 colonies were obtained on a given plate which was prepared by pour
plating with 7th dilution, determine the microbial count in terms of log cfu/ml
03
(b) Explain the role of intrinsic and extrinsic factors which affect the microbial load
in foods
04
(c) Justify how moist heat is better option for sterilization than dry heat. Explain
the terms antiseptic, aseptic, sanitization, sterilization.
07
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This post was last modified on 20 February 2020