Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2018 Winter 3rd Sem New 2131407 Basic Food Microbiology Previous Question Paper
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?III (New) EXAMINATION ? WINTER 2018
Subject Code:2131407 Date:01/12/2018
Subject Name:Basic Food Microbiology
Time:10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Draw diagrams wherever necessary.
5. Write scientific names as per the norms
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom classification based
on characteristics.
03
(b) Justify why Robert Koch is also known as Father of Modern bacteriology 04
(c) Describe the difference between gram +ve and gram ?ve bacteria. Give
example of gram +ve rod, gram +ve cocci, gram ?ve rod, gram ?ve cocci
07
Q.2 (a) Write a short note on chemical and physical methods to control
microorganisms
03
(b) Describe the difference between eukaryotic and prokaryotic cell 04
(c) Microbial growth occurs is observed as sigmoidal curve. Describe each
phase of the sigmoidal curve. During which phase the primary metabolites
are formed?
07
OR
(c) Justify he statement ?Louis Pasteur is aptly known as Father of
Microbiology
07
Q.3 (a) An apple pie sample contained 10000 cells of S. aureus. And it was kept at
room temperature for 4 hrs. What would be the generation time if the final
no. of cells is 6.5 X 10^8.
03
(b) Explain Redi?s, Needham?s and Spallanzani?s experiments. Which scientist
favored concept of biogenesis
04
(c) Using a schematic diagram, describe the steps of western blotting
technique. For which substance, northern blotting is used?
07
OR
Q.3 (a) Enlist the significance of microorganisms in agricultural microbiology 03
(b) Give example of microorganisms 1) gram positive spherical cells in chain
2) photoautotrophic bacteria 3) poisonous fungi 4) gram negative spherical
04
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Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?III (New) EXAMINATION ? WINTER 2018
Subject Code:2131407 Date:01/12/2018
Subject Name:Basic Food Microbiology
Time:10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Draw diagrams wherever necessary.
5. Write scientific names as per the norms
MARKS
Q.1 (a) Draw a flowchart to depict the scheme of five kingdom classification based
on characteristics.
03
(b) Justify why Robert Koch is also known as Father of Modern bacteriology 04
(c) Describe the difference between gram +ve and gram ?ve bacteria. Give
example of gram +ve rod, gram +ve cocci, gram ?ve rod, gram ?ve cocci
07
Q.2 (a) Write a short note on chemical and physical methods to control
microorganisms
03
(b) Describe the difference between eukaryotic and prokaryotic cell 04
(c) Microbial growth occurs is observed as sigmoidal curve. Describe each
phase of the sigmoidal curve. During which phase the primary metabolites
are formed?
07
OR
(c) Justify he statement ?Louis Pasteur is aptly known as Father of
Microbiology
07
Q.3 (a) An apple pie sample contained 10000 cells of S. aureus. And it was kept at
room temperature for 4 hrs. What would be the generation time if the final
no. of cells is 6.5 X 10^8.
03
(b) Explain Redi?s, Needham?s and Spallanzani?s experiments. Which scientist
favored concept of biogenesis
04
(c) Using a schematic diagram, describe the steps of western blotting
technique. For which substance, northern blotting is used?
07
OR
Q.3 (a) Enlist the significance of microorganisms in agricultural microbiology 03
(b) Give example of microorganisms 1) gram positive spherical cells in chain
2) photoautotrophic bacteria 3) poisonous fungi 4) gram negative spherical
04
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cell
(c) Describe PCR, its applications, advantages and disadvantages. Draw a
diagram to depict PCR cycle and enlist the ingredients used for PCR
07
Q.4 (a) Explain the formation of HFr and F? strains formed during bacterial
conjugation. Also write about the significance of HFr and F?
03
(b) Write a descriptive note on battery of tests abbreviated as IMViC. Write the
IMViC reaction for any two microorganisms
04
(c) Bacteria change from non-pathogenic strain to pathogenic strain. Explain
the concept with experiment conducted by Griffith. Also suggest an
application in reference to food industry.
07
OR
Q.4 (a) Draw a diagram to depict parts of compound bright field microscope. Why
immersion oil is necessary while using 100X objective?
03
(b) Draw a diagram to depict Holliday junction. How does it helps in
evolution?
04
(c) What is synbiotic food? Enlist any 7 fermented foods along with the starter
organism.
07
Q.5 (a) What is direct and indirect ELISA and list down applications. 03
(b) Explain the concept of reactions observed in Triple sugar iron agar test.
Suggest an application of the same.
04
(c) Virus can transfer bacterial DNA from donor bacteria to recipient bacteria.
Explain the concept in reference to lytic and lysogeny cycle.
07
OR
Q.5 (a) If 220 colonies were obtained on a given plate which was prepared by pour
plating with 7th dilution, determine the microbial count in terms of log
cfu/ml
03
(b) Explain the role of intrinsic and extrinsic factors which affect the microbial
load in foods
04
(c) Justify how moist heat is better option for sterilization than dry heat.
Explain the terms antiseptic, aseptic, sanitization, sterilization.
07
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This post was last modified on 20 February 2020