Download GTU BE/B.Tech 2018 Winter 3rd Sem New 2131407 Basic Food Microbiology Question Paper

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Seat No.:

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Subject Code: 2131407

Enrolment No.

GUJARAT TECHNOLOGICAL UNIVERSITY

BE - SEMESTER-III (New) EXAMINATION — WINTER 2018

Subject Name: Basic Food Microbiology

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Time: 10:30 AM TO 01:00 PM

Date: 01/12/2018

Total Marks: 70

Instructions:

1. Attempt all questions.

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2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
4. Draw diagrams wherever necessary.
5. Write scientific names as per the norms

Q.1

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1. Draw a flowchart to depict the scheme of five kingdom classification based on characteristics. (03 MARKS)
2. Justify why Robert Koch is also known as Father of Modern bacteriology (04 MARKS)
3. Describe the difference between gram +ve and gram –ve bacteria. Give example of gram +ve rod, gram +ve cocci, gram –ve rod, gram –ve cocci (07 MARKS)

Q.2

1. Write a short note on chemical and physical methods to control microorganisms (03 MARKS)

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2. Describe the difference between eukaryotic and prokaryotic cell (04 MARKS)
3. Microbial growth occurs is observed as sigmoidal curve. Describe each phase of the sigmoidal curve. During which phase the primary metabolites are formed? (07 MARKS)

OR

1. Justify the statement: “Louis Pasteur is aptly known as Father of Microbiology (07 MARKS)

Q.3

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1. An apple pie sample contained 10000 cells of S. aureus. And it was kept at room temperature for 4 hrs. What would be the generation time if the final no. of cells is 6.5 X 10^8. (03 MARKS)
2. Explain Redi’s, Needham’s and Spallanzani’s experiments. Which scientist favored concept of biogenesis (04 MARKS)
3. Using a schematic diagram, describe the steps of western blotting technique. For which substance, northern blotting is used? (07 MARKS)

OR

1. Enlist the significance of microorganisms in agricultural microbiology (03 MARKS)

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2. Give example of microorganisms 1) gram positive spherical cells in chain 2) photoautotrophic bacteria 3) poisonous fungi 4) gram negative spherical cell (04 MARKS)

Q.4

1. Describe PCR, its applications, advantages and disadvantages. Draw a diagram to depict PCR cycle and enlist the ingredients used for PCR (07 MARKS)
2. Explain the formation of HFr and F’ strains formed during bacterial conjugation. Also write about the significance of HFr and F’ (03 MARKS)
3. Write a descriptive note on battery of tests abbreviated as IMViC. Write the IMVIC reaction for any two microorganisms (04 MARKS)

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OR

1. Bacteria change from non-pathogenic strain to pathogenic strain. Explain the concept with experiment conducted by Griffith. Also suggest an application in reference to food industry. (07 MARKS)

Q.5

1. Draw a diagram to depict parts of compound bright field microscope. Why immersion oil is necessary while using 100X objective? (04 MARKS)
2. Draw a diagram to depict Holliday junction. How does it helps in evolution? (07 MARKS)

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OR

1. What is synbiotic food? Enlist any 7 fermented foods along with the starter organism. (03 MARKS)
2. What is direct and indirect ELISA and list down applications. (04 MARKS)
3. Explain the concept of reactions observed in Triple sugar iron agar test. Suggest an application of the same. (07 MARKS)

Q.6

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1. Virus can transfer bacterial DNA/from donor bacteria to recipient bacteria. Explain the concept in reference to lytic and lysogeny cycle. (03 MARKS)

OR

1. If 220 colonies were obtained on a given plate which was prepared by pour plating with 7th dilution, determine the microbial count in terms of log cfu/ml (04 MARKS)
2. Explain the role of intrinsic and extrinsic factors which affect the microbial load in foods (07 MARKS)

Q.7

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1. Justify how moist heat is better option for sterilization than dry heat. (03 MARKS)
2. Explain the terms antiseptic, aseptic, sanitization, sterilization. (04 MARKS)

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