Download GTU BE/B.Tech 2018 Winter 5th Sem New 2150401 Advanced Molecular Biology I Question Paper

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GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER-V (NEW) EXAMINATION - WINTER 2018

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Subject Code:2150401 Date:27/11/2018
Subject Name:Advanced Molecular Biology - 1
Time: 10:30 AM TO 01:00 PM Total Marks: 70

Instructions:

1. Attempt all questions.

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2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

MARKS

Q.1 (a) Draw and annotate the map of F factor. 03
(b) What are F prime cells? How are they generated? 04

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(c) Explain the process of DNA mobilisation from donor to recipient during conjugation. 07

Q.2 (a) Explain three different mechanism by which conjugation takes place. 03
(b) What are prerequisites for conjugation to occur? 04
(c) Compare generalised and specialised transduction. 07

OR

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(c) Explain abortive transduction with a neat diagram. 07

Q.3 (a) What is gene cloning? 03
(b) Explain U tube experiment used in discovery of transduction. 04
(c) Explain mechanism of natural competence in Haemorphilus influenzae with suitable diagram. 07

OR

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Q.3 (a) What is competence? 03
(b) What are the factors that affect mechanism of transformation. 04
(c) Explain mechanism of natural competence in Streptococcus pneumonia with suitable diagram. 07

Q.4 (a) What is biological role of Homologous Recombination? 03
(b) Explain two possible resolution fates of DSB repair pathway. 04

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(c) Explain the RecBCD pathway system of E.coli. 07

OR

Q.4 (a) What is significance of melting point Tm in PCR? 03
(b) How the restriction enzymes are named? Give an example 04
(c) Explain the RecBCD pathway system of E.coli. 07

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Q.5 (a) What are linkers and adapters? 03
(b) Explain principle working of northern hybridisation. 04
(c) Differentiate in detail between PBR322 and PUCS8 with suitable diagram 07

OR

Q.5 (a) What are palindromic sequences? 03

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(b) Explain principle and working of western hybridisation. 04
(c) Define the term PCR. Explain the principle and application of PCR. 07