Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2018 Winter 7th Sem New 2170401 Enzymes And Proteins Previous Question Paper
1
Seat No.: ________ Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER ?VII (NEW) EXAMINATION ? WINTER 2018
Subject Code: 2170401 Date: 15/11/2018
Subject Name: Enzymes and Proteins
Time: 10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
MARKS
Q.1 (a) Explain ?Lock and Key? hypothesis. 03
(b) Explain the role of entropy in enzyme catalysis. 04
(c) What is enzyme specificity? Describe different types of enzyme
specificities with suitable example.
07
Q.2 (a) Enlist six classes of enzymes. 03
(b) Explain collision theory of enzyme action. 04
(c) What is enzyme immobilization? Discuss various physical techniques of
enzyme immobilization.
07
OR
(c) Define enzyme immobilization. Discuss various chemical techniques of
enzyme immobilization.
07
Q.3 (a) Explain the effect of temperature on enzyme action. 03
(b) Write the advantages of enzyme immobilization. 04
(c) Compare the kinetics of single substrate and multi-substrate reactions. 07
OR
Q.3 (a) Explain the competitive inhibition of enzyme. 03
(b) Explain the concept of crude enzyme and pure enzyme. 04
(c) Comment on the derivation of Michaelis-Menten Equation and its
significance.
07
Q.4 (a) Give the significance of Vmax. 03
(b) How new functions evolved in proteins. 04
(c) Comment on the use of enzymes in leather and textile industries. 07
OR
Q.4 (a) Diagnostic use of enzymes. 03
(b) Allosteric regulation of enzymes. 04
(c) Comment on the production and purification of crude enzyme extracts
from plant sources.
07
Q.5 (a) Explain the modular structure of proteins. 03
(b) Methods of characterization of enzymes. 04
(c) Comment on the production and purification of crude enzyme extracts
from animal sources.
07
OR
Q.5 (a) What is protein evolution? 03
(b) Comment on the protein folding patterns. 04
(c) Comment on the production and purification of crude enzyme extracts
from microbial sources.
07
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