Download GTU BE/B.Tech 2018 Winter 7th Sem New 2170401 Enzymes And Proteins Question Paper

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GUJARAT TECHNOLOGICAL UNIVERSITY

BE - SEMESTER-VII (NEW) EXAMINATION - WINTER 2018

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Subject Code: 2170401

Subject Name: Enzymes and Proteins

Date: 15/11/2018

Time: 10:30 AM TO 01:00 PM

Total Marks: 70

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Instructions:

1. Attempt all questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

1. 1. Explain “Lock and Key” hypothesis. [03]

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   2. Explain the role of entropy in enzyme catalysis. [04]
   3. What is enzyme specificity? Describe different types of enzyme specificities with suitable example. [07]
2. 1. Enlist six classes of enzymes. [03]
   2. Explain collision theory of enzyme action. [04]

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   3. What is enzyme immobilization? Discuss various physical techniques of enzyme immobilization. [07]

   OR

   1. Define enzyme immobilization. Discuss various chemical techniques of enzyme immobilization. [07]
3. 1. Explain the effect of temperature on enzyme action. [03]

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   2. Write the advantages of enzyme immobilization. [04]
   3. Compare the kinetics of single substrate and multi-substrate reactions. [07]

   OR

   1. Explain the competitive inhibition of enzyme. [03]
   2. Explain the concept of crude enzyme and pure enzyme. [04]

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   3. Comment on the derivation of Michaelis-Menten Equation and its significance. [07]
4. 1. Give the significance of Vmax. [03]
   2. How new functions evolved in proteins. [04]
   3. Comment on the use of enzymes in leather and textile industries. [07]

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   OR

   1. Diagnostic use of enzymes. [03]
   2. Allosteric regulation of enzymes. [04]
   3. Comment on the production and purification of crude enzyme extracts from plant sources. [07]

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5. 1. Explain the modular structure of proteins. [03]
   2. Methods of characterization of enzymes. [04]
   3. Comment on the production and purification of crude enzyme extracts from animal sources. [07]

   OR

   1. What is protein evolution? [03]

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   2. Comment on the protein folding patterns. [04]
   3. Comment on the production and purification of crude enzyme extracts from microbial sources. [07]