This download link is referred from the post: GTU BE/B.Tech 2017 Winter Question Papers || Gujarat Technological University
GUJARAT TECHNOLOGICAL UNIVERSITY
BE - SEMESTER-VII (NEW) EXAMINATION — WINTER 2017
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Subject Code: 2170401 Date: 02/11/2017Subject Name: Enzymes and Proteins
Time: 10:30 AM TO 01:00 PM Total Marks: 70
Instructions:
- Attempt all questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
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| MARKS | |
| Q.1 (a) Explain “lock and key” hypothesis. | 03 |
| (b) What are the six classes of enzymes? Give suitable example. | 04 |
| (c) Describe different types of enzyme specificities with examples. | 07 |
| Q.2 (a) Explain “induced-fit” theory of enzyme action. | 03 |
| (b) Comment on the allosteric regulation of enzyme action. | 04 |
| (c) Give the derivation of Michelis-Menten Equation. | 07 |
| OR | |
| (c) Comment on the formation and stabilization of secondary structure of proteins. | 07 |
| Q.3 (a) Write a note on multi-subunit protein. | 03 |
| (b) Explain transition state theory of enzyme catalysis. | 04 |
| (c) Describe different physical methods of enzyme immobilization. | 07 |
| OR | |
| Q.3 (a) Explain the concept of active site and binding site in enzyme. | 03 |
| (b) Explain collision theory of enzyme catalysis. | 04 |
| (c) How will you obtained crude enzyme extracts from microbes? | 07 |
| Q.4 (a) Write various limitations of enzyme immobilization. | 03 |
| (b) Comment on the analysis of film and pore diffusion. | 04 |
| (c) Comment on the use of enzymes in textile & leather industries. | 07 |
| OR | |
| Q.4 (a) Write the advantages of enzyme immobilization. | 03 |
| (b) How will you immobilize invertase enzyme? | 04 |
| (c) How will you isolate and purify enzymes from plant sources? | 07 |
| Q.5 (a) How pH affects the activity of enzyme? | 03 |
| (b) Comment on the diagnostic use of enzymes. | 04 |
| (c) Comment on the survey of protein structures and functions. | 07 |
| OR | |
| Q.5 (a) How temperature affects the activity of enzyme? | 03 |
| (b) Comment on the analytical use of enzymes. | 04 |
| (c) Explain the concept of protein folding. Comment on the idea of protein design. | 07 |
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This download link is referred from the post: GTU BE/B.Tech 2017 Winter Question Papers || Gujarat Technological University