Download GTU BE/B.Tech 2017 Winter 3rd Sem Old 131402 Basic Food Microbiology Question Paper

Download GTU (Gujarat Technological University) BE/BTech (Bachelor of Engineering / Bachelor of Technology) 2017 Winter 3rd Sem Old 131402 Basic Food Microbiology Previous Question Paper

1
Seat No.: ________ Enrolment No.___________

GUJARAT TECHNOLOGICAL UNIVERSITY

BE - SEMESTER ?III (OLD) EXAMINATION ? WINTER 2017
Subject Code:131402 Date:17/11/2017

Subject Name: Basic Food Microbiology

Time: 10:30 AM to 01:00 PM Total Marks: 70

Instructions:

1. Attempt all questions.

2. Make suitable assumptions wherever necessary.

3. Figures to the right indicate full marks.

Q.1 (a) Describe the difference between gram +ve bacteria and gram ?ve bacteria. 07
(b) Describe the contributions of Louis Pasteur in field of Microbiology. 07

Q.2 (a) Describe the difference between prokaryotes and eukaryotes. 07
(b) Describe the contributions of Robert Koch. Enlist the Koch postulates which help
in establishing a causative of a particular disease.
07
OR
(b) Describe the growth phases of bacterial growth alongwith a typical growth curve
diagram.
07

Q.3 (a) Draw a diagram to depict steps of western blotting. 07
(b) What is transduction? How does specialized transduction differ from generalized
transduction?
07
OR
Q.3 (a) Draw a flowchart to depict the steps of ELISA. Write the full form of ELISA and
function of secondary antibody.
07
(b) Bacteria are transformed from R strain to S strain by transformation. Explain the
concept with experiment conducted by Griffith.
07

Q.4 (a) Describe the significance of microorganisms in food and agriculture 07
(b) Explain the Redi?s experiment. What were the critic?s comments 07
OR
Q.4 (a) Describe steps of bacterial DNA exchange by conjugation using a well labelled
diagram.
07
(b) Draw a diagram to depict a typical PCR cycle. What is the significance of primers
and Taq polymerase?
07

Q.5 (a) Describe the role of intrinsic and extrinsic parameters affecting microbial growth
in foods
07
(b) A typical bacterial cell increases from one cell to 1024 cells in 10 hours. What is
the generation time of this organism?
07
OR

Q.5 (a) Describe significance of probiotics. Name any three probiotic microorganisms
and any two probiotic food products available in Indian market.
07
(b) Explain the concept of IMViC test used for differentiating enteric bacteria 07

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