Subject Code: 131402
GUJARAT TECHNOLOGICAL UNIVERSITY
--- Content provided by FirstRanker.com ---
BE - SEMESTER-III (OLD) EXAMINATION — WINTER 2017
Subject Name: Basic Food Microbiology
Time: 10:30 AM to 01:00 PM
Instructions:
- Attempt all questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
--- Content provided by FirstRanker.com ---
Q.1 (a) Describe the difference between gram +ve bacteria and gram —ve bacteria. (07)
(b) Describe the contributions of Louis Pasteur in field of Microbiology. (07)
Q.2 (a) Describe the difference between prokaryotes and eukaryotes. (07)
--- Content provided by FirstRanker.com ---
(b) Describe the contributions of Robert Koch. Enlist the Koch postulates which help in establishing a causative of a particular disease. (07)
OR
(b) Describe the growth phases of bacterial growth along with a typical growth curve diagram. (07)
Q.3 (a) Draw a diagram to depict steps of western blotting. (07)
(b) What is transduction? How does specialized transduction differ from generalized transduction? (07)
--- Content provided by FirstRanker.com ---
OR
Q.3 (a) Draw a flowchart to depict the steps of ELISA. Write the full form of ELISA and function of secondary antibody. (07)
(b) Bacteria are transformed from R strain to S strain by transformation. Explain the concept with experiment conducted by Griffith. (07)
Q.4 (a) Describe the significance of microorganisms in food and agriculture (07)
(b) Explain the Redi’s experiment. What were the critic’s comments (07)
--- Content provided by FirstRanker.com ---
OR
Q.4 (a) Describe steps of bacterial DNA exchange by conjugation using a well labelled diagram. (07)
(b) Draw a diagram to depict a typical PCR cycle. What is the significance of primers and Taq polymerase? (07)
Q.5 (a) Describe the role of intrinsic and extrinsic parameters affecting microbial growth in foods (07)
(b) A typical bacterial cell increases from one cell to 1024 cells in 10 hours. What is the generation time of this organism? (07)
--- Content provided by FirstRanker.com ---
OR
Q.5 (a) Describe significance of probiotics. Name any three probiotic microorganisms and any two probiotic food products available in Indian market. (07)
(b) Explain the concept of IMVIC test used for differentiating enteric bacteria (07)
Date: 17/11/2017
Total Marks: 70
--- Content provided by FirstRanker.com ---
This download link is referred from the post: GTU BE/B.Tech 2017 Winter Question Papers || Gujarat Technological University
--- Content provided by FirstRanker.com ---