Download PTU B.Tech 2020 March Biotechnology 6th Sem BTBT 603 Enzymology And Enzyme Technology Question Paper

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Roll No. [ ] [ ] [ ] Total No. of Pages : 02
Total No. of Questions : 09

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B.Tech.(Biotechnology) (Sem.-6)
ENZYMOLOGY AND ENZYME TECHNOLOGY
Subject Code : BTBT-603
M.Code : 71074
Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

SECTION-A

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1. Write briefly :
   1. Classes of enzymes
   2. Enzyme assay
   3. Fischer lock and key hypothesis
   4. Lineweaver-Burk plot
   5. Eadie-Hofstee and Hanes plot

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   6. Microencapsulation
   7. Enzyme reactors
   8. Biocatalysis
   9. Monomeric enzymes
   10. Enzyme active site

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SECTION-B

2. Describe briefly industrial applications of enzymes.
3. Discuss Koshland induced-fit hypothesis.
4. Describe the covalent coupling and cross linking of enzymes.

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5. What do you understand from steady state analysis of mass transfer? Explain mass transfer in enzyme reactors.
6. Describe the Haldane relationship for reversible reactions.

SECTION-C

6. Discuss enzyme catalysis in organic media. Also highlight its challenges and future perspectives.
7. Describe the criteria used for the selection of an enzyme reactor. Describe idealized enzyme reactor systems.

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8. Describe the Henri & Michaelis-Menton equation and Briggs-Haldane modification of the Michaelis-Menton equation. Also highlight significance of Michaelis-Menton equation.

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