Download PTU B.Tech 2020 March Biotechnology 6th Sem BTBT 605 Bioanalytical Techniques Question Paper

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Roll No. [T TTTTTT] [ ] Total No. of Pages : 02
Total No. of Questions : 09

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B.Tech.(Biotechnology) (Sem.-6)
BIOANALYTICAL TECHNIQUES
Subject Code : BTBT-605
M.Code : 71076
Time : 3 Hrs. Max. Marks : 60

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INSTRUCTION TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

SECTION-A

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Q1. Answer briefly :

1. What is a Geiger-Muller counter?
2. Explain Beer-Lambert law.
3. What is retention time in chromatography?
4. Briefly explain FIGE technique.

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5. What is differential centrifugation?
6. What is isoelectric focusing?
7. Give applications of Ion-Exchange Chromatography.
8. Write short note on Polyacrylamide Gel Electrophoresis.
9. Briefly Types of radioactive decay.

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10. What is capillary electrophoresis?

SECTION-B

Q2 Describe the underlying principle of AFM. Describe the uses of AFM.

Q3 Describe the different types of columns used in gas chromatography.

Q4 Explain working principle and components of NMR. Describe its applications.

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Q5 Describe the technique of western blot in detail.

Q6 Give the classification of different types of objective lenses used in Light Microscopy. Which is the best lens and why?

SECTION-C

Q7 What is electron microscopy? How is contrast generated in specimens of electron microscopy? Compare magnification and resolution of electron microscopy and light microscopy

Q8 a) Explain the principle and working of X-Ray crystallography. Briefly describe its applications.

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b) Write principle, construction and applications of stereo microscope.

Q9 Explain the principle and working of IR spectroscopy. List the types of detector used in IR and explain them in detail.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.