Download PTU (I.K. Gujral Punjab Technical University Jalandhar (IKGPTU) ) BE/BTech Bio-Technology (Biotechnology Engineering) 2020 March 7th and 8th Sem BTBT 702 Downstream Processing Previous Question Paper
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Tech.(BT) (2012 to 2017) (Sem.?7)
DOWNSTREAM PROCESSING
Subject Code : BTBT-702
M.Code : 71844
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt ANY FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt ANY TWO questions.
SECTION-A
1. Write briefly :
a. Selection criteria for downstream processing of a fermentation product.
b. Sedimentation coefficient of biological macromolecules.
c. Define ?Selectivity Factor?.
d. Enlist various methods used for precipitation of proteins.
e. Name any two flocculating agents.
f. Salting-in and salting-out phenomenon for protein purification.
g. Isoelectric focusing.
h. Ultrafiltration.
i. Define the term ?Partition Coefficient?.
j. Principle of capillary electrophoresis.
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1 | M - 7 1 8 4 4 ( S 2 ) - 2 0 6
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Tech.(BT) (2012 to 2017) (Sem.?7)
DOWNSTREAM PROCESSING
Subject Code : BTBT-702
M.Code : 71844
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt ANY FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt ANY TWO questions.
SECTION-A
1. Write briefly :
a. Selection criteria for downstream processing of a fermentation product.
b. Sedimentation coefficient of biological macromolecules.
c. Define ?Selectivity Factor?.
d. Enlist various methods used for precipitation of proteins.
e. Name any two flocculating agents.
f. Salting-in and salting-out phenomenon for protein purification.
g. Isoelectric focusing.
h. Ultrafiltration.
i. Define the term ?Partition Coefficient?.
j. Principle of capillary electrophoresis.
2 | M - 7 1 8 4 4 ( S 2 ) - 2 0 6
SECTION-B
2. Describe the process design criteria for various classes of bio-products.
3. Explain the effect of pressure drop on cake resistance in a batch filtration. What is the
effect of compressible cake formation on filtration process?
4. Explain the principle of centrifugal separation. Mention the important features of tubular
bowl centrifuge along with a neat sketch.
5. What are adsorption isotherms? Explain the adsorption in fixed beds.
6. A colloidal suspension of clay became clear upon being allowed to stand undisturbed for
5 minutes in ethanol at 20?C. The height of the suspension in the vessel was 45 cm and
the specific gravity of montmorillonite is known to be 1.92 g/cm
3
. The viscosity of
ethanol at 20?C was calculated to be 1.2 centipoises. Estimate the average diameter of
clay particles.
SECTION-C
7. Define ?crystallization?. Explain various phases of a crystallization process. Derive the
relationship between size and population density of crystals formed during continuous
crystallization.
8. a. Write a complete note on working principle, instrumentation and characteristics of a
chromatographic techniques exclusive for protein separation. Also mention some
matrices used for large-scale chromatography.
b. Define ion-exchange and Principle of ion-exchange chromatography. Mention the
applications of ion- exchangers.
9. Comment on following (also provide the necessary sketch) :
a. Two phase extraction process
b. Dialysis
NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any
page of Answer Sheet will lead to UMC against the Student.
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This post was last modified on 21 March 2020