Download OU B.Sc 2nd Year 2019 Microbiology Question Bank Question Paper

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Time:2llrsMax.Marks:25
Note:Eachstudenthastoperformoneexperimentandfourspotters
I.ExperimentQuestion(l2Marks)
l.DemonsimteStreak/spreadplatetechniqueforisolatingpurecultures.
.Abacteriologicalculturemediumwithcolonies

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is
providedto
you.
linumerzttethe
bacterialnumberandreportthenumberin(Fl-Ufgittufstail.

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.Identifytheunknuw-nstigarspresentinthegivenmixturebyqualitativeanalysis.
Identifytheunknownaminoacidspresentinthegivenmixturebyqualitativeainalysis.
IdentifytheunknownaminoacidsinthegivenmixtureusingPaper(Ihromatography
IdentifytheunknownsugarsinthegivenmixtureusingPaperChromatography
FindouttheAbsorptionNlaximzitxfthcgivencolnurcdsolution.

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.VerifyBeer-LumbcrtslawforcolouredsolutionllSingcalorimeter.
9.De?nebuffer.Prepareabuffersolutionwithspecificpllandreportitspll.
IQ
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ll.SpecimensforSpotting(4x2=8Marks)

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l.NutrientAgarplate
2.NutrientagarSlant
3.Agardeeptubes
4.PDA(Withlabeling)
5.Autoclave

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6Hotairoven
7.Fillrzilionapparatus
8.Streakplate
9.
Spreadplate

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1t.Mineraloiloverlaytube
ll.Colorillleter
l2.Papercliromatogram(aminoacids/sugars)
13.pHmeter-Estrips
M.lluller(labelled)

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Ill.RecordandViva(5Marks)
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