Download OU B.Sc 4th Year 2019 Microbiology QB Question Paper

Time:2hoursMax.Nlarks?

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l.ExperimentQuestions(12I?arks)
~1.Findnu:thequantityofProteinpresentinthegivensampleby3iuretll.o\vry's
wcdandplotastwitlardgraphof[rrtrlcinbycalorimetry(Theexaminer\vil|
provideunknownsampleandimcessaryreagents).

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Z.FindvulLiceamountofDNApresentinlllugivenseuuplubyl?grheuylans-Inc
mclivutlandplntastandardgrapho.?i)'I\'/\bycalorimetry(lhct-"xatnincrwill
provideunknownsampleandnecessaryreagents).
3.Plotastandardgraphnl?RNAbycalorimetryusingOrcittolmethodandfindout
thequantityofRNApresentinthegiver.sample(Theexamine."willprovide

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tuiknoxvttsampleandnecessaryreagents).
lsoiategenomicI).\JAfromthegivensample.Obseweandreporttheresult.
Asampleisprovidedtoyou.Demonstrateelectrophorelicseparationbymixing
Agarosegelelectrophoresisandinterpretyourresuil.
6.ProblemstaintedtoDNA,RNAcharaetcrisliux.TranscriptionandTmnslaliun

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(Theexaminerscantareparcprobl?m?tobesolvedbyritestudents].
5".?-
ll.Spottings(4x2=8Marks)
7.Calorimeter
8.Cuvette

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9.Micropipette
l?.(Yentrifuge
.__-~~~~~'"'11.PCRkit
?QLE?l2.Geleieutroplzoresisunit
lK-DPHrezigentwithlabel

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l4.'l'l:Jbuffer
IS.DiagrammaticalrepresentationofLaoopercn
l6.Biuretreagentwithlabel
17.Orcinolreagentwithlabel
l8.GraphshowingtheestimationofDNAwithlabel

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l9.GraphshowingtheestimationofRNAwithlabel-
20.Graphshowingtheestimationof?utcinwithlalzcl
III.Record&viva(SMarks)

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