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Subject Title: Molecular Biology and r-DNA Technology Prepared by: Arshiya Jabeen
Year: II Semester: III Updated on: 28.12.2020
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Unit - I: Genome organization and DNA replication
Essay questions:
- What are the experiments that prove DNA as genetic material? Explain
- Write an essay on RNA as genetic material.
- Explain about Organization of Eukaryotic nuclear genome.
- What is Genome organization? Explain organization of Mitochondrial genome.
- What is DNA replication? Write about DNA replication enzymes involved in the replication of DNA.
- Explain about Replication of nuclear genome of Eukaryotes
- What is Mutation? Explain types of mutations.
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Short questions:
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- Replication of Prokaryotic genome
- Organization of Chloroplast genome
- Origin of replication fork
- spontaneous, induced mutations
- Organization of Prokaryotic genome
- Griffith’s experiment
- RNA as genetic material-TMV
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Unit - II: Gene expression in Prokaryotes and Eukaryotes
Essay questions:
- Describe the structure of eukaryotic gene.
- What are RNA Polymerases? Explain their structural and functional features.
- What is Genetic code? Explain its properties.
- Write in detail about Deciphering of genetic code.
- Define Genetic code. Explain about Wobble hypothesis.
- Explain in detail about Transcription mechanism in prokaryotes.
- Explain in detail about Transcription mechanism in eukaryotes.
- What is Translation. Explain the mechanism of Translation in detail.
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Short questions:
- Structure of prokaryotic gene
- Structure and functions of prokaryotic RNA polymerase
- Genetic code
- Deciphering of genetic code
- Wobble hypothesis
- rho independent & rho dependent termination
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Unit -III : Gene Regulation in Prokaryotes and Eukaryotes
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Essay questions:
- What is lac operon. Explain about lac operon in detail
- Write an essay on tryptophan operon
- What are Restriction endonucleases. Explain
- What are Post-transcriptional modifications? Explain
- Describe Post translational modifications.
- Explain about Gal regulation in yeast.
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Short questions:
- Alternate splicing
- Polyadenylation
- Capping
- Glycosylation
- Ubiquitination
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Unit -IV : Recombinant DNA technology
Essay questions:
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- What is cloning? Explain about enzymes useful in molecular cloning.
- What are Cloning Vectors. Explain various types of Cloning Vectors.
- Explain about vectors used for library preparation.
- What do you mean by gene transfer. Explain various Gene transfer techniques.
- Describe different methods for selection of recombinant clones.
- What is rDNA technology? What are the applications of recombinant DNA technology?
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Short questions:
- DNA Polymerase
- DNA ligases
- PBR 3
- Shuttle vectors
- Bacteriophage
- Cosmids
- BAC, YAC
- Gene transfer techniques: Chemical methods
- Biological methods
- Colony hybridization
- Applications of recombinant DNA technologies-pharmaceutics and medicine
- Recombinant DNA-technology in Agriculture, diagnostics
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