Subject Title: Immunotechnology
Year: II Semester: IV
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Updated on: 23.03.2019
Unit - I: Antibody Assays- Principle, Methodology & Applications
Essay Answer Type
- Explain about precipitation and agglutination reactions and their applications.
- Write briefly about western blotting and its applications.
- What is mean by immunohistochemistry? Explain methodology and applications of immunohistochemistry.
- Explain briefly about ELISA.
- What is the principle involved in immunofluorescent assay and explain methodology.
- Describe radial immunodiffusion and its applications.
- Discuss briefly about Immunoelectrophoresis and its applications.
- What is RIA? Explain procedure and applications.
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Short Answer Type
- Write applications of ELISA
- Agglutination reactions
- Immunodiffusion
- Applications of western blotting
- Principle involved in immunoelectrophoresis
- Immunohistochemistry
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Unit - II: Cellular Assays- Principle, Methodology & Applications
Essay Answer Type
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- Explain procedure of differential count in human peripheral blood.
- Write the principles of separation of mononuclear cells from human peripheral blood.
- Explain about lymphocyte transformation assay.
- Write the method for separation of T & B cells from human blood.
- Write about cell mediated cytotoxicity.
- Discuss about micro cytotoxicity and its role in HLA typing.
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Short Answer Type
- Applications of lymphocyte transformation assay
- Cell mediated cytotoxicity
- T and B lymphocytes
- What is HLA typing
- Write principles of micro cytotoxicity
- Explain method of differential count
- Write the principle involved in separation of mononuclear cells.
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