Download AKTU B-Tech 3rd Sem 2015-2016 NBT 303 Biochemistry Question Paper

Download AKTU (Dr. A.P.J. Abdul Kalam Technical University (AKTU), formerly Uttar Pradesh Technical University (UPTU) B-Tech 3rd Semester (Third Semester) 2015-2016 NBT 303 Biochemistry Question Paper

Printed Pages: 4 ' 277 I ' ' NBT?303
(Following Paper ID and Roll No. to be filled in your
AnsWer Book)
Roll No.
B. TECH;
(SEM. 111) THEORY EXAMINATION, 2015-16
BIOCHEMISTRY
[Time:3 hours] A [MaximumMarkszlool'
J
Section-A
Note: Attempt all parts. All parts carry equal marks. Write
answer of each part in short.
1. Put True (T) or F else (F ). in the space : (2x10=20)
(a) The nucleosome consists of core of eight sub-units
of protein called histones containing DNA. ()
(b) The naturally occuring small peptide hormone
secreted by pituitary gland is called insulin. ( )
(c) The electrophoresis in presence of SDS separates
' the protein on the basis of charge. ( )
1200 (1) ' P.T.O.

(d)
i (6)
I200
(f)
(g)
(h)
(j),
The amino terminal residue in a polypeptide chain
can be determined by 1, ?uoro, 2, 4 dinitrobenzene
()
Myoglobin was the ?rst protein for which the three
dimensional structure was worked out by X-ray
crystallography. ( )
Ceruloplasmin is a protein which transports zinc.
0
Hypo?xanthine is derived from adenine by
replacement of its amino group with methoxy
groun 1" ( )
Pyrimidines and purines which contain -NH2 group
are weakly basic. ( )
?B?structure is one of themost important secondaxy
structures in protein. ( )
Base composition of DNA varies from species to
species. ( )
(2) NBT?303
SectiOn-B
Attempt any ?ve questions from this section : (10 x 5 = 50)
2.
Explain the principle of Capillary Electrophoresis.
How is Ramchandran Plot used in determining Secondary
structure of proteins ??
How glucokinase and insulin regulate blood glucose
? levels ?
What is Henderson Hasselbach equation ?
Explain the differences of denaturation of protein by urea
~ and quanidine hydro chloride.
De?ne the reasons for Wobble effect.
Cation-exchange resins be used for puri?cation of
? protein.
1200
What do you understand by homologous proteins.
Explain giving suitable examples.
(3) _ p.10.

Section-C
Attempt any two questions from this section. (15x2=30)
10. Describe hemoglobin structure and its role in oxygen
' transport and release.
11. Describe two examples of diseases caused by altered
protein cqnformalion.
12. Explain De novo salvage pathways of pyrimidine
nucleotides.
1200 _ . (4) . NBT-303