Download AKTU B-Tech 4th Sem 2015-16 NBT 403EBT Enzyme Engineering Question Paper

Download AKTU (Dr. A.P.J. Abdul Kalam Technical University (AKTU), formerly Uttar Pradesh Technical University (UPTU) B-Tech 4th Semester (Fourth Semester) 2015-16 NBT 403EBT Enzyme Engineering Question Paper

Printed Pages: 4 NBT-403/EBT-401/BT-404
(Following Paper ID and Roll No. to be filled in your
Answer Books)
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B.TECH.
Theory Examination (Semester?lV) 2015-16
ENZYME ENGINEERING
Time : 3 Hours Max. Marks : 100
Scction?A
Q.l Attempt all parts. All parts carry equal marks. Write
answer of each part in short. (2X10=20)
(a) What is the importance of prosthetic group in an
enzyme?
(b) What is the unit of Michaelis Menten constant?
(0) Sketch lineweaver-burk plot showing the effect of
competitive inhibition.
(d) Differentiate activation and deactivation energy of
enzymes.
(1) P.T.O.
2805/235/33/825

(e) De?ne the unit of enzyme activity.
(0 What is enzyme dialysis in af?nity chromatography?
(g) What is the difference between adsorption and ab-
sorption?
(h) What is encapsulation of enzyme?
(i) What is the difference between packed bed reactor
(PBR) and ?uidized bed reactor (FBR)?
0) Write down the element of biosensors.
Section-B
QZ. Attempt any five parts. All parts cany equal marks:
(5X10=50)
(a) Derive a Michaelis-Menten equation for enzyme cata-
lyzed reaction.
(b) Describe the co-operative behaviour of hemoglobin
through concerted and sequential model.
(0) Describe diagrammatically puri?cation of enzyme
through af?nity chromatography.
(2)
2805/235/33/825
(d)
(e)
(f)
(g)
(h)
What is adsorption immobilization? Explain
advantages and disadvantages in brief.
Explain immobilized enzyme reactor CSTR with neat
and clean ?gure.
Write the step involved in extraction of crude
enzyme from plant source.
What is product inhibition? Explain negative and
positive feedback inhibition with appropriate
example.
Write the applications of immobilized enzyme
system.
Section-C
Attempt any two questions from this section.
(2x15=30)
QS. Explain the matrix entrapment of enzyme in brief. Write
advantages and disadvantages of immobilization of enzymes by
matrix entrapment. Give two examples of immobilization of
enzymes by matrix entrapment.
(3) P.T.O.
2805/235/33/825

Q4. Explain two methods for the assay of total protein.
Q5. Derive Michaelis-Menten equation for uncompetitive
inhibition. Draw the lineweaver burk plot showing the effect
of uncompetitive inhibition.
\
(4)
2805/235/33/825