Download PTU B.Sc-Agriculture 2020 March 7th Sem 77103 Molecular Biotechnology And Genomics Question Paper

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Roll No. [ TTTTTT] [ ] Total No. of Pages : 02

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INSTRUCTIONS TO CANDIDATES :

1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students have to attempt any TWO questions.

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SECTION-A

Q1. Write briefly :

1. Genome
2. BLAST and its types
3. pBR322

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4. Palindromic sequences
5. Shuttle Vectors
6. LOD score
7. NCBI
8. Isoschizomers with example

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9. ISSRs
10. Linkers and Adaptors

SECTION-B

Q2. a) Define Gene Cloning and enlist its basic steps.

Q3. b) What are the functions of the following in a gene cloning experiment?

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1. Type II endonucleases
2. Alkaline phosphatases

Q4. a) Define RFLPs. What is the basis of polymorphism using RFLP markers?

Q5. b) What do you understand by polymorphic and monomorphic marker?

Q6. c) Are SSRs dominant or co-dominant markers?

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Q7. Define Proteome. What are the major steps that constitute a proteome analysis?

Q8. What is the principle of Sanger’s sequencing method and what are its advantages over Maxam and Gilbert sequencing technique?

Q9. Define Molecular map. Discuss briefly about the various segregating populations that could be used to construct a linkage map.

SECTION-C

Discuss in details about the Principle, Advantages, Limitations and Applications of any two Hybridization techniques.

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What is a cDNA clone? Enlist basic steps involved in cDNA cloning. Describe in details the synthesis of first and second strand of cDNA.

a) Define PCR and its various components. Diagrammatically represent different stages of PCR.

b) Name any two bioinformatics tools for PCR primers designing.

c) Give any three reasons that could lead to PCR failure.

NOTE : Disclosure of Identity by writing Mobile No. or Making of passing request on any page of Answer Sheet will lead to UMC against the Student.

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