Download PTU B.Sc-BT 2020 March 5th Sem 47056 Animal Cell Culture And Biotechnology Question Paper

Download PTU (I.K. Gujral Punjab Technical University Jalandhar (IKGPTU) B.Sc. in Biotechnology 2020 March Previous Question Papers

1 | M-47056 (S2)-1600
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc.(BT) (2014 to 2017) (Sem.?5)
ANIMAL CELL CULTURE AND BIOTECHNOLOGY
Subject Code : BSBT-301
M.Code : 47056
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
Q1. Define briefly :
a) Monolayer culture
b) Histotypic culture
c) Hybrid Antibodies
d) Cell Cultures
e) Cell line
f) Biolistic gun method
g) Recombinant proteins
h) Generation number
i) Transfection
j) In vitro fertilization

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1 | M-47056 (S2)-1600
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc.(BT) (2014 to 2017) (Sem.?5)
ANIMAL CELL CULTURE AND BIOTECHNOLOGY
Subject Code : BSBT-301
M.Code : 47056
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
Q1. Define briefly :
a) Monolayer culture
b) Histotypic culture
c) Hybrid Antibodies
d) Cell Cultures
e) Cell line
f) Biolistic gun method
g) Recombinant proteins
h) Generation number
i) Transfection
j) In vitro fertilization

2 | M-47056 (S2)-1600
SECTION-B
Q2. Differentiate between Suspension and Monolayer cultures.
Q3. Write a note on immobilized cultures.
Q4. Discuss steps in initiation of primary culture.
Q5. Write a note on Embryo transfer.
Q6. Discuss about natural and artificial media used for cell culture.

SECTION-C
Q7. Elaborate on production of monoclonal antibodies.
Q8. What are transgenic animals? Discuss various methods of transfection.
Q9. Write about bioethical considerations and guidelines for cell culture research.










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