Download PTU B.Sc-MLS 2020 March 3rd Sem 48110 Analytical Biochemistry Question Paper

Download PTU (I.K. Gujral Punjab Technical University Jalandhar (IKGPTU) B.Sc MLS (B.Sc Medical Laboratory Sciences) 2020 March Previous Question Papers

1 | M-48110 (S2)-1279
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc.(MLS) (2014 to 2017) (Sem.?3)
ANALYTICAL BIOCHEMISTRY
Subject Code : BMLS-305
M.Code : 48110
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
1) Answer briefly :
a) Give applications of column chromatography.
b) What is a standard graph?
c) What is monochromatic light?
d) Give applications of agarose gel electrophoresis.
e) List some limitations of thin layer chromatography.
f) What is a cation?
g) Define Excited State of an atom.
h) Explain the term absorbance.
i) What is the full form of SDS-PAGE?
j) What is mobile phase in chromatography?


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1 | M-48110 (S2)-1279
Roll No. Total No. of Pages : 02
Total No. of Questions : 09
B.Sc.(MLS) (2014 to 2017) (Sem.?3)
ANALYTICAL BIOCHEMISTRY
Subject Code : BMLS-305
M.Code : 48110
Time : 3 Hrs. Max. Marks : 60
INSTRUCTION TO CANDIDATES :
1. SECTION-A is COMPULSORY consisting of TEN questions carrying TWO marks
each.
2. SECTION-B contains FIVE questions carrying FIVE marks each and students
have to attempt any FOUR questions.
3. SECTION-C contains THREE questions carrying TEN marks each and students
have to attempt any TWO questions.

SECTION-A
1) Answer briefly :
a) Give applications of column chromatography.
b) What is a standard graph?
c) What is monochromatic light?
d) Give applications of agarose gel electrophoresis.
e) List some limitations of thin layer chromatography.
f) What is a cation?
g) Define Excited State of an atom.
h) Explain the term absorbance.
i) What is the full form of SDS-PAGE?
j) What is mobile phase in chromatography?


2 | M-48110 (S2)-1279
SECTION-B
2) What is the role of gas chromatography in a biochemistry lab? What are its limitations?
3) Discuss the factors affecting migration of charged particles during electrophoresis.
4) Write a short note on cation and anion exchangers.
5) Describe the principle, method and application of affinity chromatography.
6) What is native PAGE and what are its applications?

SECTION-C
7) Give the principle, instrumentation and applications of paper electrophoresis.
8) Explain the principle and working of a spectrophotometer. List some of its merits.
9) What are different parts of a flame emission spectroscope? Describe the principle and
working of this instrument.








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