Download GTU B.Tech 2020 Winter 7th Sem 2170401 Enzymes And Proteins Question Paper

Download GTU (Gujarat Technological University Ahmedabad) B.Tech/BE (Bachelor of Technology/ Bachelor of Engineering) 2020 Winter 7th Sem 2170401 Enzymes And Proteins Previous Question Paper

Seat No.: ________
Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE- SEMESTER?VII (NEW) EXAMINATION ? WINTER 2020
Subject Code:2170401 Date:19/01/2021
Subject Name:Enzymes and Proteins
Time:10:30 AM TO 12:30 PM Total Marks: 56
Instructions:
1. Attempt any FOUR questions out of EIGHT questions.

2. Make suitable assumptions wherever necessary.

3. Figures to the right indicate full marks.
MARKS
Q.1 (a) Explain the role of entropy in catalysis of enzyme.
03
(b) Explain about specificity of enzymes.
04
(c) Explain the method of classification of enzyme.
07
Q.2 (a) Discuss the significance of Vmax.
03
(b) Explain features and working of active site.
04
(c) Compare different plots and modifications used to overcome limitations
07
of Michealis-Menten plot with equation.
Q.3 (a) What do you mean by enthalpy, entropy and free energy.
03
(b) Write Michaelis-Menten equation & Write assumptions for derivation of
04
Michaelis-Menten equation.
(c) Explain the concept of standard free energy.
07
Q.4 (a) Write a note on Sangers method for sequencing genes.
03
(b) What are various features of protein protein interface?
04
(c) Discuss in detail about the factors that affect the rate of chemical reaction.
07
Q.5 (a) Which method can give the highest resolution of protein structure? Write
03
a brief note about the same.
(b) Compare NMR and X-Ray crystallography as protein structure tools.
04
(c) Write a note on CD-Spectroscopy, Maldi-Tof and at least one method for
07
understanding protein primary structure.
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Q.6 (a) Write note with examples on enzymes from plant source , animal source
03
and microbial source.
(b) Write a note about commercial applications of enzymes in at least two
04
different industries.
(c) Write a note on allosteric activation and inhibition as a means for control
07
of protein activity.
Q.7 (a) Define enzyme activity, effectiveness factor and domains.
03
(b) What is Anfinsen's hypothesis? How it was demonstrated?
04
(c) Write a note on enzyme immobilization.
07
Q.8 (a) Effect of denaturants on protein structure.
03
(b) How folding funnels describe folding phenomenon?
04
(c) Explain GroEL GroES system and its function.
07

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