Download GTU B.Tech 2020 Winter 7th Sem 2170407 Biochemical Engineering I Question Paper

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GUJARAT TECHNOLOGICAL UNIVERSITY
BE- SEMESTER-VII (NEW) EXAMINATION - WINTER 2020

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Subject Code:2170407 Date:25/01/2021
Subject Name:Biochemical Engineering-I
Time:10:30 AM TO 12:30 PM Total Marks: 56

Instructions:

1. Attempt any FOUR questions out of EIGHT questions.

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2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

MARKS

Q.1 (a) How are cells classified? 03
(b) What is central dogma of life? Explain in brief. 04

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(c) Derive the equation for batch reaction time required to reduce the 07
substrate concentration from sp to sfin an enzymatic process.

Q.2 (a) What do you mean by recycle stream, bypass stream and purge stream? 03
(b) A continuous process is set up for treatment of wastewater. Each day, 04
10⁶ kg cellulose and 10⁶ kg bacteria enter in the feed stream, while 10⁴

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kg cellulose and 1.5 x 10⁴ kg bacteria leave in the effluent. The rate of
cellulose digestion by the bacteria is 7 x 10⁴ kg d^-1. The rate of bacterial
growth is 2 x 10⁴ kg d^-1; the rate of cell death by lysis is 5 x 10³ kg d^-1.
Write balances for
Cellulose and bacteria in the system.

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(c) Explain different modes of sterilization of liquids. 07

Q.3 (a) What do you mean by state properties? 03
(b) Draw a neat diagram of stirred tank reactor-with proper annotations. 04
(c) Classify the fluids according to their rheological behaviour. Also show 07
their flow curve.

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Q.4 (a) Compare packed bed, fluidized bed and trickel bed reactors. 03
(b) What is Reynold’s number? Give its equation and significance. 04
(c) Explain various factors affecting broth viscosity. 07

Q.5 (a) Explain the terms:bubble formation, gas dispersion and coalescence 03
(b) Explain Michaelis Menten Kinetics. 04

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(c) Explain role of diffusion in a bioprocess. 07

Q.6 (a) What is total reaction rate, volumetric reaction rate and specific reaction 03
rate?
(b) Compare Lineweaver-Burk plot, Eadie-Hofstee plot, Langmuir plot and 04
Direct liner plot.

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(c) Explain film theory in detail. 07

Q.7 (a) How the Cell Senses Its Extracellular Environment? 03
(b) What do you mean by overall yield, instantaneous yield, theoretical and 04
observed yield?
(c) Draw and explain Typical batch growth curve and state values of µ in 07

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different phases.

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Q.8 (a) What is µmax and ks? Give relation between them. 03
(b) What is k_La? What are ways to measure it? 04
(c) Explain principle and working of Cone-and-Plate Viscometer. 07

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