GTU B.Tech 2020 Winter Question Papers || Gujarat Technological University
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GUJARAT TECHNOLOGICAL UNIVERSITY
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BE- SEMESTER-V (NEW) EXAMINATION - WINTER 2020
Subject Code:3150401 Date:27/01/2021
Subject Name:Advanced Molecular Biology
Time:10:30 AM TO 12:30 PM Total Marks:56
Instructions:
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- Attempt any FOUR questions out of EIGHT questions.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
| Marks | ||
| Q.1 | (a) Explain the concept of a quantitative trait locus. | 03 |
| (b) Compare abiotic and biotic stress that plants undergo and explain in a paragraph any biotechnology method that is employed to overcome such stress elements. | 04 | |
| (c) Using marker assisted selection is better than traditional selective breeding. Explain this statement. | 07 | |
| Q.2 | (a) Explain the process of conjugation. | 03 |
| (b) What are transposons? Explain the mechanism of any one type of transposon. | 04 | |
| (c) Evaluate the figure given in the question and determine the type of molecular marker being used in the figure. Explain the process of using this marker to distinguish between individuals. | 07 | |
| Q.3 | (a) Explain the role of Homologous recombination for generation of transgenic mice. | 03 |
| (b) Differentiate between positive and negative regulation of operon. | 04 | |
| (c) Gene A is suspected to be a tumor suppressor gene. You are given a Lab with mice and access to Crispr-Cas 9 guide RNA cassette ready for expression in mice, how will you evaluate the function of Gene A? | 07 | |
| Q.4 | (a) Why is the C.elegans or the worm model used for studying developmental biology? What are the different stages of development of a C. elegans worm? | 03 |
| (b) Differentiate between transgenic mice and knockout mice. | 04 | |
| (c) What is tmRNA? Explain mechanism of transcriptional regulation by tmRNA. | 07 | |
| Q.5 | (a) Explain Intein-based vectors in detail. | 03 |
| (b) Explain blunt and cohesive ends. | 04 | |
| (c) What is genetic engineering? Explain different tools used in genetic engineering. | 07 | |
| Q.6 | (a) Compare cDNA and genomic library. | 03 |
| (b) Explain type and properties of restriction endonuclease. | 04 | |
| (c) What is vector? Give basic properties of vector. Explain artificial chromosome (BAC, YAC) as a vector. | 07 | |
| Q.7 | (a) What is constitutive and inducible gene? | 03 |
| (b) What are inclusion bodies? How can inclusion bodies formation be reduced? | 04 | |
| (c) Explain mechanism of attenuation in trp operon. | 07 | |
| Q.8 | (a) Explain the term operon. Explain its basic structure. | 03 |
| (b) What is difference between phagemid and cosmid vector? | 04 | |
| (c) What is plasmid? Explain any one plasmid vector along with its screening process. | 07 |
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