Download GTU (Gujarat Technological University Ahmedabad) B.Tech/BE (Bachelor of Technology/ Bachelor of Engineering) 2020 Winter 5th Sem 3150401 Advanced Molecular Biology Previous Question Paper
Enrolment No.___________
GUJARAT TECHNOLOGICAL UNIVERSITY
BE- SEMESTER?V (NEW) EXAMINATION ? WINTER 2020
Subject Code:3150401 Date:27/01/2021
Subject Name:Advanced Molecular Biology
Time:10:30 AM TO 12:30 PM Total Marks:56
Instructions:
1. Attempt any FOUR questions out of EIGHT questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.
Marks
Q.1 (a) Explain the concept of a quantitative trait locus.
03
(b) Compare abiotic and biotic stress that plants undergo and explain in a
04
paragraph any biotechnology method that is employed to overcome such
stress elements.
(c) Using marker assisted selection is better than traditional selective breeding.
07
Explain this statement.
Q.2 (a) Explain the process of conjugation.
03
(b) What are transposons? Explain the mechanism of any one type of transposon.
04
(c)
07
Evaluate the figure given in the question and determine the type of molecular
marker being used in the figure. Explain the process of using this marker to
distinguish between individuals.
Q.3 (a) Explain the role of Homologous recombination for generation of of
03
transgenic mice.
(b) Differentiate between positive and negative regulation of operon.
04
(c) Gene A is suspected to be a tumor suppressor gene. You are given a Lab with
07
mice and access to Crispr-Cas 9 guide RNA cassette ready for expression in
mice, how will you evaluate the function of Gene A?
Q.4 (a) Why is the C.elegans or the worm model used for studying developmental
03
biology? What are the different stages of development of a C. elegans worm?
(b) Differentiate between transgenic mice and knockout mice.
04
(c) What is tmRNA? Explain mechanism of transcriptional regulation by
07
tmRNA.
Q.5 (a) Explain Intein-based vectors in detail.
03
(b) Explain blunt and cohesive ends.
04
(c) What is genetic engineering? Explain different tools used in genetic
07
engineering.
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Q.6 (a) Compare cDNA and genomic library.
03
(b) Explain type and properties of restriction endonuclease.
04
(c) What is vector? Give basic properties of vector. Explain artificial
07
chromosome (BAC, YAC) as a vector.
Q.7 (a) What is constitutive and inducible gene?
03
(b) What are inclusion bodies? How can inclusion bodies formation be reduced?
04
(c) Explain mechanism of attenuation in trp operon.
07
Q.8 (a) Explain the term operon. Explain its basic structure.
03
(b) What is difference between phagemid and cosmid vector?
04
(c) What is plasmid? Explain any one plasmid vector along with its screening
07
process.
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This post was last modified on 04 March 2021