Seat No.: Enrolment No.
GUJARAT TECHNOLOGICAL UNIVERSITY
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B.Pharm - SEMESTER-V « EXAMINATION — WINTER -2020Subject Code:2250002 Date: 05/01/2021
Subject Name: Pharmaceutical Microbiology &Biotechnology-I
Time: 10:30AM TO 12:30PM Total Marks: 54
Instructions:
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- Attempt any THREE questions from Q-1 to Q-6.
- Q.7 is compulsory to attempt.
- Make suitable assumptions wherever necessary.
- Figures to the right indicate full marks.
Q.1 | (a) Define microbiology. Discuss the various scope of microbiology. | 06 |
Q.2 | (a) Discuss principle, working and applications of TEM. | 05 |
Q.3 | (a) Differentiate: Prokaryotes and eukaryotes, Bacteria and virus. | 05 |
Q.4 | (a) Give the suitable condition for growth of following: Autotrophs, Chemolithotrophs, Microaerophiles, Psychrophiles, Basophiles, Obligates anaerobes. | 06 |
Q.5 | (a) Differentiate: spore and capsule, Gm+ve and Gm —ve bacteria. | 05 |
Q.6 | (a) Write a note on bacterial flagella. | 05 |
Q.7 | (a) Define and Classify culture media. Write a note on Enriched media. | 06 |
(a) What are FTM and SCM? Write a note on-aerobic and anaerobic media. | 05 | |
(a) Define total count and viable count. Discuss any one method used for viable counting of E.coli. | 05 | |
(a) Explain D-value, F-value, Z-value; Q10Value, SAL, Bio burden. | 06 | |
(a) Write a note on Cold sterilization methods. | 05 | |
(a) Comment on followings:-(i) Superheated steam is more active than saturated steam. (i1) Roll tube method cannot be used for viable count of anaerobic organism. | 05 | |
(a) Suggest suitable sterilization method for Oily injections, Boric acid, Transfusion sets, Petri plates, Ethyl oleate and Syringes. | 06 | |
(a) Differentiate: tyndallization and pasteurization, Sterilization and disinfection. | 05 | |
(a) Write a note on biological indicators for monitoring of sterilization. | 05 | |
(a) Enlist the various evaluation tests for disinfectant. Write a note on Riedel Walker coefficient test. | 06 | |
(a) Write a note on QAC and halide class of disinfectants. | 05 | |
(a) Discuss principle and procedure involved in staining for mycobacterium species. | 05 | |
(a) Define immobilization. Enlist methods of immobilization and write any one in detail. --- Content provided by FirstRanker.com --- ORDiscuss in detail about genetic code and its properties | 06 | |
(a) OR Write a note on DNA replication. | 06 |
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