Download GTU B.Pharma 2019 Winter 6th Sem 2260002 Pharmaceutical Microbiology And Biotechnology Ii Question Paper

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Instructions:

1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

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1. (a) Give the flow sheet of tetracycline production, isolation and recovery by fermentation process 06
2. (b) Enumerate different types of mutations. Explain in detail any one of them. 05
3. (c) Briefly explain the difference between Generalized and Specialized Transduction. 05

1. (a) Discuss in detail tuberculin test and schick test 06
2. (b) Write note on secondary defense mechanism. 05

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3. (c) Briefly explain the activase enzymes. 05

1. (a) What is monoclonal antibody? Discuss the production of monoclonal antibodies. 06
2. (b) Write method for Collection, processing and storage of human fibrinogen 05
Fibrinogen
3. (c) Explain the Replica Plating Technique to detect and isolate mutants. 05

1. (a) What is toxoids? Describe preparation, standardization and storage of toxoids containing vaccines. 06

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2. (b) Write a detail note on new generation vaccines with respect to AIDS and Malaria. 05
3. (c) What is Gene cloning? Give its importance. 05

1. (a) What is protoplast fusion? Explain the methods of protoplast fusion 06
2. (b) Write a note on human normal immunoglobulin 05
3. (c) Give the comparison between Western, Northern and Southern blot technique 05

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1. (a) Explain the fermentation process of alcohol production. 06
2. (b) Explain microbiological assay of vitamin and amino acid. 05
3. (c) What is sterility testing? Explain the sterility testing of surgical product 05

1. (a) Explain detailed production and recovery of citric acid. 06
2. (b) State differences between cellular and humoral immunity. 05

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3. (c) Write note on culture media used in sterility testing. 05

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