Download GTU B.Pharma 2019 Summer 8th Sem 2280015 Genetic Engineering And Gene Therapy Question Paper

Download GTU (Gujarat Technological University Ahmedabad) B.Pharma (Bachelor of Pharmacy) 2019 Summer 8th Sem 2280015 Genetic Engineering And Gene Therapy Previous Question Paper

Seat No.: _____ Enrolment No. _____________
GUJARAT TECHNOLOGICAL UNIVERSITY
B.PHARM - SEMESTER? 8- EXAMINATION ? SUMMER -2019
Subject Code:2280015
Date: 18-05-2019
Subject Name: GENETIC ENGINEERING AND GENE THERAPY
Time: 10:30 AM TO 01:30 PM
Total Marks: 80
Instructions:


1. Attempt any five questions.
2. Make suitable assumptions wherever necessary.
3. Figures to the right indicate full marks.

Q.1 (a) What do you mean by Gene cloning? Explain the steps involved with clean and
06
labelled diagram.

(b) Explain the process of Transcription in detail with clean and labelled diagram.
05

(c) Write a note on applications of rDNA technology.
05



Q.2 (a) Explain the methods used for isolation of DNA with suitable diagram.
06

(b) Write a note on restriction enzymes their types.
05

(c) Discuss on DNA delivery methods.
05



Q.3 (a) What is PCR? Explain the cycle and write the applications in detail.
06

(b) What is HUMULIN? How it is produced using rDNA technology.
05

(c) Discuss DNA vaccine in detail.
05



Q.4 (a) What are monocloanl Antibodies? How they are produced? Write the
06
applications of Monoclonal Antibodies.

(b) Discuss Transgenic plants in detail. Write the applications of Transgenic plants. 05

(c) what do you mean by Gene therapy? Explain the types with suitable examples.
05



Q.5 (a) Write a note on DNA sequencing methods. Explain the Maxam Gilbert
06
methods for DNA sequencing with suitable diagram.

(b) Explain in detail about Transgenic animals. Write their applications.
05

(c) What do you mean by cDNA? How it is synthesized?
05



Q. 6 (a) What are vectors? Write their characteristics. Explain about PBR322 in detail.
06

(b) What are blotting techniques? Explain Southern blotting in detail with suitable
05
diagram.

(c) Explain about bacteriophage with labelled diagram.
05



Q.7 (a) Explain the applications of Gene therapy in hematopoietic disorders.
06

(b) What do you mean by RNA? Explain the types with their functions.
05

(c) Write a note on Anti sense therapy with suitable diagram.
05
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